Experimental infection of cynomolgus macaques with Ebola-Reston filoviruses from the 1989-1990 U.S. epizootic.
Experimental infection of cynomolgus macaques with Ebola-Reston filoviruses from the 1989-1990 U.S. epizootic.
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1989-1990 年美国动物流行病的埃博拉-雷斯顿丝状病毒对食蟹猴的实验性感染。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
C. Peters
中科院分区：
文献类型：
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作者：
P. Jahrling;T. Geisbert;N. Jaax;M. Hanes;T. Ksiazek;T. Ksiazek;C. Peters;C. Peters
这项研究描述了埃博拉-雷斯顿(EBO-R)亚型埃博拉病毒对实验感染食蟹猴的致病作用。EBO-R在猕猴中的病程与人类疾病非常相似，与EBO-Zaire和EBO-苏丹感染后猕猴的实验疾病非常相似。实验中感染EBO-R的食蟹猴出现食欲不振、偶尔流鼻涕、脾肿大、面部点状出血和静脉穿刺点严重皮下出血，类似于人类埃博拉热。6只感染EBO-R的猴子中有5只在接种后8至14天死亡。其中一人存活下来，并产生了针对EBO-R的高滴度中和抗体。这五只患了重病的猴子在各种身体分泌物中传播了传染性病毒。此外，在肺泡间质细胞中可见丰富的病毒，在肺泡中可见游离的病毒，这表明有可能产生传染性气溶胶。因此，预防接触丝状病毒感染的灵长类动物，包括人类，似乎是谨慎的。本实验证明EBO-R对猕猴具有致死性，能够引发和维持猕猴的流行性传染病。对该动物模型的进一步研究应有助于制定有效的埃博拉出血热免疫、治疗和控制策略。
This study describes the pathogenesis of the Ebola-Reston (EBO-R) subtype of Ebola virus for experimentally infected cynomolgus monkeys. The disease course of EBO-R in macaques was very similar to human disease and to experimental diseases in macaques following EBO-Zaire and EBO-Sudan infections. Cynomolgus monkeys infected with EBO-R in this experiment developed anorexia, occasional nasal discharge, and splenomegaly, petechial facial hemorrhages and severe subcutaneous hemorrhages in venipuncture sites, similar to human Ebola fever. Five of the six EBO-R infected monkeys died, 8 to 14 days after inoculation. One survived and developed high titered neutralizing antibodies specific for EBO-R. The five acutely ill monkeys shed infectious virus in various bodily secretions. Further, abundant virus was visualized in alveolar interstitial cells and free in the alveoli suggesting the potential for generating infectious aerosols. Thus, taking precautions against aerosol exposures to filovirus infected primates, including humans, seems prudent. This experiment demonstrated that EBO-R was lethal for macaques and was capable of initiating and sustaining the monkey epizootic. Further investigation of this animal model should facilitate development of effective immunization, treatment, and control strategies for Ebola hemorrhagic fever.