Outstanding Survival and Regeneration Process by the Use of Intelligent Acellular Dermal Matrices and Mesenchymal Stem Cells in a Burn Pig Model
Outstanding Survival and Regeneration Process by the Use of Intelligent Acellular Dermal Matrices and Mesenchymal Stem Cells in a Burn Pig Model
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DOI：
10.1016/j.transproceed.2010.09.132
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发表时间：
2010-12-01
影响因子：
0.9
通讯作者：
Maceira, A.
中科院分区：
文献类型：
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作者：
Mansilla, E.;Spretz, R.;Maceira, A.
采用猪大面积深度烧伤模型，研究了骨髓间充质干细胞（MSCs）和脱细胞猪真皮基质诱导的再生过程，脱细胞猪真皮基质通过与负载生长因子（粒细胞-巨噬细胞集落刺激因子和表皮生长因子）的可生物降解纳米纤维结合并涂覆抗CD 44单克隆抗体（智能脱细胞真皮基质，IADMs）而变得智能化。这些IADM被专门设计为作为新的生物支架整合在伤口床中，并通过抗CD 44抗体特异性招募和附着循环和/或外部应用的MSC，同时递送精确量的生长因子。通过这种方式，修复过程以及美学和功能结果在我们的烧伤模型中得到了增强。动物存活，伤口完全闭合，皮肤完全再生，没有太多疤痕。令人惊讶的是，尽管烧伤的严重性和深度，毛囊和其他皮肤附属物仍然发育。即使是烧伤的肌肉和肋骨，在研究结束时似乎也经历了再生过程。基于这些发现，我们提出了使用IADM和自体、同种异体或异种MSC，作为未来治疗大面积烧伤和可能的其他皮肤病和美容人类疾病的新范例。
A pig model with a deep large burn was used to study the regeneration process induced by mesenchymal stem cells (MSCs) and acellular pig dermal matrices, made intelligent by the combination with biodegradable nanofibers loaded with growth factors (granulocite-macrophage colony-stimulating factor and epidermal growth factor) and coated with the anti-CD44 monoclonal antibody (intelligent acellular dermal matrices, IADMs). These IADMs are specially designed to integrate in the wound bed as new biological scaffolds as well as to specifically recruit and attach circulating and/or externally applied MSCs through the anti-CD44 antibody while delivering precise amounts of growth factors. In this way, the reparative process as well as the aesthetic and functional results were enhanced in our burn model. The animal survived, the wound was completely closed, and total regeneration of the skin was obtained without much scarring. Surprisingly, hair follicles and other skin appendages developed despite the severity and deepness of the burn. Even burned muscles and ribs seemed to have undergone a regenerative process by the end of the study. Based on these findings, we have proposed the use of IADMs and autologous, allogeneic or xenogeneic MSCs, as a new paradigm for the future treatment of large burns and probably other dermatological and cosmetic human conditions.