CELL-SUSPENSION CULTURES OF MACLURA-POMIFERA .3. COMPARISON BETWEEN METABOLITE PRODUCTIONS IN CELL-CULTURE AND IN WHOLE-PLANT OF MACLURA-POMIFERA

CELL-SUSPENSION CULTURES OF MACLURA-POMIFERA .3. COMPARISON BETWEEN METABOLITE PRODUCTIONS IN CELL-CULTURE AND IN WHOLE-PLANT OF MACLURA-POMIFERA
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DOI:
10.1016/0031-9422(94)00971-u
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发表时间:
1995-06-01
期刊:
影响因子:
3.8
通讯作者:
BOTTA, B
BOTTA, B
中科院分区:
生物学2区
文献类型:
--
作者:
DELLEMONACHE, G;DE ROSA, MC;BOTTA, B

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植物组织培养显示出与亲本植物不同的代谢产物积累模式。从愈伤组织和细胞培养物中分离到三萜类和类黄酮类化合物,但没有发现在整个植物中发现的口山酮和二苯乙烯类化合物。在黄酮类化合物中,异黄酮类化合物主要由悬浮细胞产生,但异黄酮类化合物的戊烯取代基仅位于A环,异黄酮类化合物不具有果实分离产物中的3‘,4’-二羟基取代模式。从愈伤组织和细胞培养物中还分离到一种新的预烯基化的6-脱氧查尔酮。
Plant tissue cultures of Maclura pomifera showed a metabolite accumulation pattern which was both quantitatively and qualitatively different from that of the parent plant. Triterpenes and flavonoids were isolated from callus and cell cultures, however, xanthones and stilbenes, which have been reported in the whole plant, were not found. Among the flavonoids, flavones and flavanones were produced preferentially by the suspended cells, but with the prenyl substituents exclusively on ring A, while the isoflavones did not show the 3',4'-dihydroxyl substitution pattern found in the products isolated from fruits. A new prenylated 6-deoxychalcone was also isolated from the callus and cell cultures.