GeoChip as a metagenomics tool to analyze the microbial gene diversity along an elevation gradient.
GeoChip as a metagenomics tool to analyze the microbial gene diversity along an elevation gradient.
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GeoChip 作为宏基因组学工具来分析沿海拔梯度的微生物基因多样性
DOI：
10.1016/j.gdata.2014.06.003
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Yang Y
中科院分区：
文献类型：
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作者：
Gao Y;Wang S;Xu D;Yu H;Wu L;Lin Q;Hu Y;Li X;He Z;Deng Y;Zhou J;Yang Y
To examine microbial responses to climate change, we used a microarray-based metagenomics tool named GeoChip 4.0 to profile soil microbial functional genes along four sites/elevations of a Tibetan mountainous grassland. We found that microbial communities differed among four elevations. Soil pH, temperature, NH4+–N and vegetation diversity were four major attributes affecting soil microbial communities. Here we describe in details the experiment design, the data normalization process, soil and vegetation analyses associated with the study published on ISME Journal in 2014 [1], whose raw data have been uploaded to Gene Expression Omnibus (accession number GSM1185243).
