Sequences in the gag-pol-5'env region of avian leukosis viruses confer the ability to induce osteopetrosis.
Sequences in the gag-pol-5'env region of avian leukosis viruses confer the ability to induce osteopetrosis.
复制标题
禽白血病病毒的gag-pol-5env区域中的序列赋予诱导骨硬化症的能力。
DOI：
10.1016/0042-6822(85)90204-1
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Robinson,HL
中科院分区：
文献类型：
--
作者：
Shank,PR;Schatz,PJ;Jensen,LM;Tsichlis,PN;Coffin,JM;Robinson,HL
编码三种E亚群禽白血病病毒基因组的DNA序列已被分子克隆。从这些克隆DNA之一（pRAV-0）中回收的病毒不引起骨石化病，而从第二个（λ NY 203）中回收的病毒引起迟发性骨石化病，从第三个（λNTRE-2）中回收的病毒引起中发性骨石化病。将克隆的病毒DNA的限制性内切酶片段用于构建重组病毒，用于检测gag-pol-5′ env和3′env-LTR序列在骨硬化症诱导中的作用。致病性试验结果表明，gag-pol-5′ env序列具有诱发骨石化病的能力，而3′ env-LTR序列影响骨石化病的发病时间和严重程度。
DNA sequences encoding the genomes of three subgroup E avian leukosis viruses have been molecularly cloned. Virus recovered from one of these cloned DNAs (pRAV-0) caused no osteopetrosis while virus recovered from the second (λNY203) caused late onset osteopetrosis and virus recovered from the third (λNTRE-2) caused intermediate onset osteopetrosis. Restriction endonuclease fragments of the cloned viral DNAs were used to construct recombinant viruses that could be used to test for the role ofgag-pol-5′envand 3′env-LTR sequences in the induction of osteopetrosis. The results of the pathogenicity tests indicate thatgag-pol-5′envsequences confer the ability to induce osteopetrosis while 3′env-LTR sequences influence the time of onset and the severity of osteopetrosis.