Spatial variability of microbial richness and diversity and relationships with soil organic carbon, texture and structure across an agricultural field
Spatial variability of microbial richness and diversity and relationships with soil organic carbon, texture and structure across an agricultural field
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DOI：
10.1016/j.apsoil.2016.03.004
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发表时间：
2016-07-01
影响因子：
4.8
通讯作者：
de Jonge, Lis W.
中科院分区：
文献类型：
--
作者：
Naveed, Muhammad;Herath, Lasantha;de Jonge, Lis W.
尽管土壤微生物在提供各种生态系统功能和服务方面发挥着关键作用，但我们才刚刚开始了解微生物群落是如何由各种土壤物理性质和环境因素塑造的。在这项研究中，我们整合了60个地点的15 × 15米的网格在农业领域的细菌和真菌和土壤物理测量：（1）表征微生物丰富度和多样性的空间变化，（2）确定土壤物理参数，最强烈的相关性。研究田位于Silstrup丹麦，面积超过1.69公顷，向北倾斜1-2度。通过将50-mL无菌塑料小瓶推入土壤中来提取用于454-焦磷酸测序的土壤样品。散装土壤样品用于测量pH值，有机碳和土壤质地。完整的100 cm（3）土芯，一式三份收集，用于测量土壤水分特征曲线。在整个领域，细菌丰富度（OTU的数量）和多样性指数（香农多样性和系统发育多样性）表现出很强的空间自相关性。OTU的真菌数量在田间非常相似，因此没有观察到它们的空间自相关。真菌香农多样性略有增加，从南到北，空间自相关的距离大于100米。粘粒与有机碳（n）的比值被认为是细菌丰富度和多样性指数的最佳预测因子。无论是有机碳还是粘土含量与真菌丰富度和多样性指数显着相关。对于土壤结构参数，pF范围为5-6.8（参数B）的土壤持水率与细菌和真菌香农氏菌均显著相关。大孔（> 30 μ m）和总孔隙度（phi）的数量只与真菌香农多样性显着相关。这些结果表明，微生物群落的变化不是随机的，但在田间尺度上与有机碳，粘粒含量和土壤水分特性的变化密切相关。(C)2016爱思唯尔B. V.保留所有权利。
Although soil microorganisms play a pivotal role in delivering various ecosystem functions and services, we are only beginning to understand how microbial communities are shaped by various soil physical properties and environmental factors. In this study, we integrate metabarcoding of bacteria and fungi and soil physical measurements at 60 locations on a 15 x 15 m grid in an agricultural field to: (1) characterize the spatial variation of microbial richness and diversity, and (2) identify the soil physical parameters that are most strongly correlated with them. The study field is located in Silstrup Denmark, extends over 1.69 ha, and slopes 1-2 degrees to the north. Soil samples for 454-pyrosequencing were extracted by pushing 50-mL sterilized plastic vials into the soil. Bulk soil samples were used for the measurement of pH, organic carbon, and soil texture. Intact 100 cm(3) soil cores, collected in triplicate, were used for measuring the soil water characteristic curve. Across the field, bacterial richness (number of OTUs) and diversity indices (Shannon diversity and phylogenetic diversity) showed strong spatial autocorrelations. Fungal number of OTUs was quite similar over the field and thus no spatial autocorrelation was observed for them. Fungal Shannon diversity slightly increased from south to north, with spatial autocorrelation for distances larger than 100 m. The ratio of clay to organic carbon (n) was found to be the best predictor of bacterial richness and diversity indices. Neither organic carbon nor clay content was significantly correlated with fungal richness and diversity indices. For soil structural parameters, soil water retention in the pF range 5-6.8 (parameter B) was significantly correlated with both bacterial and fungal Shannon diversities. Amount of macropores (> 30 mu m) and total porosity (phi) were only significantly correlated with fungal Shannon diversity. These results suggest that variation in microbial communities is not random but strongly related with variations in organic carbon, clay content, and soil water characteristics at the field scale. (C) 2016 Elsevier B.V. All rights reserved.