The steady-state rate of ATP synthesis in the perfused rat heart measured by 31P NMR saturation transfer.
The steady-state rate of ATP synthesis in the perfused rat heart measured by 31P NMR saturation transfer.
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DOI：
10.1016/0006-291x(81)90915-3
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发表时间：
1981-12
影响因子：
3.1
通讯作者：
P. Matthews;J. Bland;D. Gadian;G. Radda
中科院分区：
文献类型：
--
作者：
P. Matthews;J. Bland;D. Gadian;G. Radda
用~（31）P核磁共振饱和传递法测定了离体Langendorff灌流大鼠心脏ATP合成的稳态速率。在37°C和70 cm H2O的灌注压下，该值为2.8 ± 0.3（n=5 ± S.E.M.）μmol.s−1。（g.干重）-1。在相同的实验中测得的肌酸磷酸激酶的活性为14.6 ± 1.0 μ mol.s-1。（g.干重）-1。根据ATP合成速率和单独测量的耗氧量，我们计算出完整组织中表观线粒体ADP：O比率为3.5 ± 0.8。
The steady-state rate of ATP synthesis in the isolated, Langendorff-perfused rat heart was determined using a31P NMR saturation transfer method. At 37°C and a perfusion pressure of 70 cm H2O the value is 2.8 ± 0.3 (n=5 ± S.E.M.) μmol.s−1. (g. dry wt.)−1. The activity of creatine phosphokinase measured in the same experiments was 14.6 ± 1.0 μ mol.s−1.(g. dry wt.)−1. From the rate of ATP synthesis and the separately measured oxygen consumption we calculated an apparent mitochondrial ADP:O ratio of 3.5 ± 0.8 in the intact tissue.