THE DEGRADATION SIGNAL IN A SHORT-LIVED PROTEIN
THE DEGRADATION SIGNAL IN A SHORT-LIVED PROTEIN
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DOI：
10.1016/0092-8674(89)90635-1
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发表时间：
1989-03-24
期刊：
影响因子：
64.5
通讯作者：
VARSHAVSKY, A
中科院分区：
文献类型：
--
作者：
BACHMAIR, A;VARSHAVSKY, A
我们以前的工作表明，短寿命蛋白的氨基末端残基是蛋白质降解信号的不同组成部分。为了定义完整的信号，在酿酒酵母及其体内半衰期中，否则具有不同延伸的二氢二氢还原酶测试蛋白和“稳定”或“稳定”或“破坏”氨基末端残基。氨基末端降解信号显示出两个不同的决定因素。以前发现的一种是蛋白质的氨基末端残基。在本工作中确定的第二个决定因素是一种特定的赖氨酸残基，其在降解信号中的功能不取决于残基附近的独特氨基酸序列。第二个决定因素的机理意义是通过发现的发现，即在有针对性的短寿命蛋白中，一系列分支的泛素 - 泛素 - 泛素结合物局限于在当前工作中已作为第二次决定的赖氨酸残基，作为赖氨酸残基的第二个决定性。降解信号。
Our previous work has shown that the amino-terminal residue of a short-lived protein is a distinct component of the protein''s degradation signal. To define the complete signal, otherwise identical dihydrofolate reductase test proteins bearing different extensions and either a "stabilizing" or a "destablizing" amino-terminal residue were expressed in the yeast Saccharomyces cerevisiae and their in vivo half-lives compared. The amino-terminal degradation signal is shown to comprise two distinct determinants. One, discovered previously, is the protein''s amino-terminal residue. The second determinant, identified in the present work, is a specific lysine residue whose function in the degradation signal is not dependent on the unique amino acid sequences in the vicinity of the residue. The mechanistic significance of the second determinant is illuminated by the finding that in a targeted, short-lived protein, a chain of branched ubiquitin-ubiquitin conjugates is confined to a lysine residue that has been idenified in the present work as the second determinant of the degradation signal.