The effect of exogenous calcium on mitochondria, respiratory metabolism enzymes and ion transport in cucumber roots under hypoxia.
The effect of exogenous calcium on mitochondria, respiratory metabolism enzymes and ion transport in cucumber roots under hypoxia.
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DOI：
10.1038/srep11391
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发表时间：
2015-08-25
影响因子：
4.6
通讯作者：
Guo S
中科院分区：
文献类型：
--
作者：
He L;Li B;Lu X;Yuan L;Yang Y;Yuan Y;Du J;Guo S
低氧诱导植物胁迫，特别是在水培黄瓜植物中。在植物中，钙参与胁迫信号的传递和生长。本研究旨在探讨外源钙对黄瓜（Cucumis sativus L.）CV.津春2号）在低氧条件下生根。我们的实验表明，外源性钙降低活性氧（ROS）的水平，并在缺氧条件下增加线粒体的抗氧化酶的活性。外源性钙还能促进参与糖酵解和三羧酸（TCA）循环的酶的积累。利用荧光和超微结构细胞化学方法，观察了外源钙通过提高质膜H ~+-ATPase、液泡膜H ~+-ATPase和H ~+-PPase的活性，增加根细胞内Ca ~（2+）和K ~+浓度。总体而言，我们的研究结果表明，低氧胁迫对根系有直接和实质性的影响。外源钙改善了黄瓜根系的代谢和离子转运，从而提高了黄瓜的耐缺氧能力。
Hypoxia induces plant stress, particularly in cucumber plants under hydroponic culture. In plants, calcium is involved in stress signal transmission and growth. The ultimate goal of this study was to shed light on the mechanisms underlying the effects of exogenous calcium on the mitochondrial antioxidant system, the activity of respiratory metabolism enzymes, and ion transport in cucumber (Cucumis sativus L. cv. Jinchun No. 2) roots under hypoxic conditions. Our experiments revealed that exogenous calcium reduces the level of reactive oxygen species (ROS) and increases the activity of antioxidant enzymes in mitochondria under hypoxia. Exogenous calcium also enhances the accumulation of enzymes involved in glycolysis and the tricarboxylic acid (TCA) cycle. We utilized fluorescence and ultrastructural cytochemistry methods to observe that exogenous calcium increases the concentrations of Ca2+ and K+ in root cells by increasing the activity of plasma membrane (PM) H+-ATPase and tonoplast H+-ATPase and H+-PPase. Overall, our results suggest that hypoxic stress has an immediate and substantial effect on roots. Exogenous calcium improves metabolism and ion transport in cucumber roots, thereby increasing hypoxia tolerance in cucumber.