Molecular genotyping of dengue viruses by phylogenetic analysis of the sequences of individual genes
Molecular genotyping of dengue viruses by phylogenetic analysis of the sequences of individual genes
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DOI：
10.1016/j.jviromet.2008.07.021
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发表时间：
2008-12-01
影响因子：
3.1
通讯作者：
Zhang, C.
中科院分区：
文献类型：
--
作者：
Klungthong, C.;Putnak, R.;Zhang, C.
近年来，随着病毒遗传多样性的增加，四种血清型登革热病毒（DENV）的流行率急剧上升。登革热病毒的演变对其对人类的毒力和全世界登革热的流行病学产生了重大影响。为了开展疾病监测，了解登革热病毒的进化及其对病毒传播和疾病的影响，需要一种高效、准确的基因型鉴定方法。病毒基因序列的系统发育分析是最常用的方法，包膜(E)基因是最常选择的目标。为了确定哪些基因可能是DENV基因分型的合适靶点，研究人员对56个地理上不同的DENV菌株的10个编码基因和t -非翻译区（TNTR）进行了系统发育分析。这些都反映在11个最大似然系统发育树中。根据支持主要节点的bootstrap值（大于90%），确定了各血清型的最佳靶基因：DENV-1为除非结构基因4A （NS4A）外的所有编码基因序列，DENV-2为PrM/M、E、NS1、NS3、NS4A和NS5， DENV-3为所有编码基因和TNTR， DENV-4为C、PrM/M、E、NS1、NS2A、NS2B、NS4A和NS5。Elsevier B.V.出版
The prevalence of four serotypes of dengue virus (DENV) has risen dramatically in recent years accompanied by an increase in viral genetic diversity. The evolution of DENV has had a major impact on their virulence for humans and on the epidemiology of dengue disease around the world. In order to perform disease surveillance and understand DENV evolution and its effects on virus transmission and disease, an efficient and accurate method for genotype identification is required. Phylogenetic analysis of viral gene sequences is the method used most commonly, with envelope (E) gene the most frequently selected target. To determine which gene might be suitable targets for genotyping DENV, phylogenetic analysis was performed on 10 individual coding genes plus the T-non-translated region (TNTR) for 56 geographically divergent DENV strains representing all identified genotypes. These were reflected in eleven maximum likelihood phylogenetic trees. Based on the bootstrap values (over 90%) supporting the major nodes, the best target genes were identified for each serotype: for DENV-1, the sequences of all coding genes except non-structural gene 4A (NS4A), for DENV-2, PrM/M, E, NS1, NS3, NS4A and NS5, for DENV-3, all coding genes and the TNTR, and for DENV-4, C, PrM/M, E, NS1, NS2A, NS2B, NS4A and NS5. Published by Elsevier B.V.