SUBLETHAL DAMAGE, POTENTIALLY LETHAL DAMAGE, AND CHROMOSOMAL-ABERRATIONS IN MAMMALIAN-CELLS EXPOSED TO IONIZING-RADIATIONS
SUBLETHAL DAMAGE, POTENTIALLY LETHAL DAMAGE, AND CHROMOSOMAL-ABERRATIONS IN MAMMALIAN-CELLS EXPOSED TO IONIZING-RADIATIONS
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DOI：
10.1016/0360-3016(91)90320-4
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发表时间：
1991-11-01
影响因子：
7
通讯作者：
BEDFORD, JS
中科院分区：
文献类型：
--
作者：
BEDFORD, JS
亚致死性和潜在致死性损伤是抽象术语，其起源和定义均未提及登记此类辐射损伤的分子和亚细胞实体。 染色体片段丢失和电离辐射杀伤细胞之间的因果关系的建立，沿着大量的辐射细胞遗传学知识，允许在假设变得可检验的背景下定义这些术语，并且更有可能朝着更好地理解这些现象的方向发展。 因此，最符合观察结果的最简单假设如下。 大多数畸变是交换类型，需要相互作用以形成染色体的两个断裂区域之间的交换，即断裂对。 很少有单一的断裂不能重新连接或恢复，所以绝大多数是亚致命的。 如果另一个亚致死断裂发生在两者可能相互作用的某个范围内，则任何这样的亚致死断裂都可能成为潜在的致死断裂对。 其中错误修复事件导致致死性无着丝粒片段产生交换的断裂对的比例可以根据处理条件而改变。 这种情况改变了“PLD修复”和“PLD固定”之间的平衡。“关于控制放射敏感性的研究集中在修复过程的差异上，但是在操作中相同的修复过程但具有不同的固定条件下，辐射反应也可能出现很大的差异。
Sublethal and potentially lethal damage are abstract terms that originated and were defined without reference to molecular and subcellular entities in which such radiation damage was registered. The establishment of a cause-and-effect relationship between chromosome fragment loss and cell killing by ionizing radiations, along with a substantial body of knowledge in radiation cytogenetics, allows a definition of these terms in a context where hypotheses become testable and progress toward a better understanding of these phenomena is more likely. Accordingly, the simplest hypothesis which best fits the observations is as follows. Most aberrations are exchange types requiring an interaction to form the exchange between two broken regions of a chromosome or chromosomes, that is, a break-pair. Very few single breaks fail to rejoin or restitute, so the vast majority are sublethal. Any such sublethal break may become a potentially lethal break-pair if another sublethal break occurs within some range where it is possible for the two to interact. The proportion of break-pairs in which a mis-repair event results in a lethal acentric fragment-producing exchange, can be altered depending on treatment conditions. Such conditions change the balance between "PLD repair" and "PLD fixation." Studies on the control of radiosensitivity have focused on differences in repair processes, but large differences in radiation response may just as well occur with identical repair processes in operation but with different conditions of fixation.