Semen granulocyte elastase: its relevance for the diagnosis and prognosis of silent genital tract inflammation.
Semen granulocyte elastase: its relevance for the diagnosis and prognosis of silent genital tract inflammation.
复制标题
DOI：
10.1093/humrep/15.9.1978
复制
发表时间：
2000-09
影响因子：
6.1
通讯作者：
B. Zorn;I. Virant-Klun;H. Meden‐Vrtovec
中科院分区：
文献类型：
--
作者：
B. Zorn;I. Virant-Klun;H. Meden‐Vrtovec
通过免疫测定法对 312 名不孕不育门诊就诊男性的精浆中的弹性蛋白酶抑制剂复合物进行了评估。使用受试者工作特征 (ROC) 曲线分析，截止值 > 或 = 290 ng/ml 的弹性蛋白酶在检测白细胞精子症 (> 1x10(6) 白细胞/ml) 定义的生殖道炎症方面是有效的（敏感性 79.5%，特异性 74.4%）。 292 名不育男性中弹性蛋白酶升高的患病率 (34%) 显着高于 20 名生育男性中观察到的弹性蛋白酶升高的患病率 (5%) (P: = 0.02)。此外，在 264 名没有白细胞精子症的男性中，有 66 名 (25%) 观察到高弹性蛋白酶浓度 (> 或 =290 ng/ml)。弹性蛋白酶浓度与患者年龄（r = 0.202，P：< 0.0001）和白细胞数量（r = 0.330，P：< 0.0001）之间存在显着正相关。弹性蛋白酶浓度与精液量（r=-0.146，P：=0.01）和单链DNA精子百分比（r=-0.194，P：=0.024）呈负相关，但弹性蛋白酶与精子活性氧产生量不存在相关性。较高的精液弹性蛋白酶浓度与女性伴侣的输卵管损伤显着相关（P：<0.001）。诺氟沙星抗生素治疗后，60 名接受治疗的患者中有 15 名 (25%) 观察到弹性蛋白酶浓度下降。伴侣的输卵管损伤对抗生素治疗的反应产生了负面影响。总之，粒细胞弹性蛋白酶是夫妻无症状生殖道炎症的可靠筛查试验。弹性蛋白酶抑制剂复合物可能对减少精子 DNA 变性具有保护作用。
Elastase-inhibitor complex was assessed by immunoassay in the seminal plasma of 312 men attending the outpatient infertility clinic. Using receiver operating characteristic (ROC) curve analysis, elastase at the cut-off value of > or =290 ng/ml was shown to be efficient (sensitivity 79.5%, specificity 74.4%) in the detection of genital tract inflammation as defined by leukocytospermia (>1x10(6) leukocytes/ml). The prevalence of increased elastase in 292 infertile men was significantly higher (34%) as compared with that (5%) observed in 20 fertile men (P: = 0.02). Moreover, high elastase concentration (> or =290 ng/ml) was observed in 66 of the 264 men (25%) without leukocytospermia. A significant positive correlation was found between elastase concentration and patient age (r = 0.202, P: < 0.0001) and the number of leukocytes (r = 0.330, P: < 0.0001). A negative correlation was found between elastase concentration and semen volume (r = -0.146, P: = 0.01) and the percentage of spermatozoa with single-stranded DNA (r = -0.194, P: = 0.024), but there was no correlation between elastase and sperm reactive oxygen species production. A higher seminal elastase concentration was significantly associated with tubal damage in female partners (P: < 0.001). After norfloxacine antibiotic therapy, decrease in elastase concentration was observed in 15 (25%) of the 60 treated patients. Tubal damage in the partner negatively affected the response to antibiotic therapy. In conclusion, granulocyte elastase is a reliable screening test for silent genital tract inflammation of the couple. The elastase-inhibitor complex may have a protective effect in reducing sperm DNA denaturation.