Functionalized Polymeric Nanoparticles Loaded with Indocyanine Green as Theranostic Materials for Targeted Molecular Near Infrared Fluorescence Imaging and Photothermal Destruction of Ovarian Cancer Cells
Functionalized Polymeric Nanoparticles Loaded with Indocyanine Green as Theranostic Materials for Targeted Molecular Near Infrared Fluorescence Imaging and Photothermal Destruction of Ovarian Cancer Cells
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DOI：
10.1002/lsm.22269
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发表时间：
2014-09-01
影响因子：
2.4
通讯作者：
Anvari, Bahman
中科院分区：
文献类型：
--
作者：
Bahmani, Baharak;Guerrero, Yadir;Anvari, Bahman
背景和目的：卵巢癌是女性生殖系统最致命的恶性肿瘤。识别和破坏所有卵巢肿瘤结节的能力可能对预防肿瘤复发和患者生存产生巨大影响。本研究的目的是研究纳米结构系统用于人表皮生长因子受体2（HER2）过表达（作为卵巢癌细胞的生物标志物）的组合近红外（NIR）荧光成像和这些细胞的体外光热破坏的有效性。材料和方法：纳米结构的系统由近红外染料，吲哚菁绿色（ICG），封装在聚（烯丙基胺）盐酸盐链与磷酸钠离子交联。构建体的表面通过共价连接的聚乙二醇和使用还原胺化方法的抗HER2的单克隆抗体官能化。我们使用动态光散射，吸收和荧光光谱的物理表征的结构。使用流式细胞术和荧光显微术来研究构建体针对SKOV 3和OVCAR 3卵巢癌细胞系的分子靶向和成像能力，所述细胞系分别具有相对高和低的HER2受体表达水平。结果：流式细胞术结果显示，功能化纳米结构比未封装ICG和非功能化结构更有效地靶向HER2受体（P
Background and Objectives: Ovarian cancer remains the deadliest malignancy of the female reproductive system. The ability to identify and destroy all ovarian tumor nodules may have a termendous impact on preventing tumor recurrence, and patient survival. The objective of this study is to investigate the effectiveness of a nano-structured system for combined near infrared (NIR) fluorescence imaging of human epidermal growth factor receptor-2 (HER2) over-expression, as a biomarker of ovarian cancer cells, and photothermal destruction of these cells in vitro.Materials and Methods: The nano-structured system consists of the near infrared dye, indocyanine green (ICG), encapsulated within poly(allylamine) hydrochloride chains cross-linked ionically with sodium phosphate. The surface of the construct is functionalized by covalently attached polyethylene glycol, and monoclonal antibodies against HER2 using reducitve amination methods. We use dynamic light scattering, and absorption and fluorescence spectroscopy for phyiscal characterization of the constructs. Flow cytometry and fluorescence microscopy are used to investigate molecular targeting and imaging capabilities of the constructs against SKOV3 and OVCAR3 ovarian cancer cell lines, which have relatively high and low expression levels of the HER2 receptor, respectively. Continuous NIR laser irradiation at 808 nm is used to investigating the utility of the constructs in mediating photothermal destruction of SKOV3 cells.Results: Flow cytometry results indicate that the functionalized nano-constructs are more effective in targeting the HER2 receptor than non-encapsulated ICG and non-functionlaized constructs (P