A rice jacalin-related mannose-binding lectin gene, OsJRL, enhances Escherichia coli viability under high salinity stress and improves salinity tolerance of rice
A rice jacalin-related mannose-binding lectin gene, OsJRL, enhances Escherichia coli viability under high salinity stress and improves salinity tolerance of rice
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DOI：
10.1111/plb.12514
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发表时间：
2017-03-01
期刊：
影响因子：
3.9
通讯作者：
Xu, Z.
中科院分区：
文献类型：
--
作者：
He, X.;Li, L.;Xu, Z.
盐度是最常见的非生物胁迫之一，严重影响植物的生产力和品质。虽然植物凝集素被认为在病原体攻击时植物防御信号中起重要作用，但对植物凝集素在抗逆性中的作用知之甚少。从水稻“Nipponbare”中克隆并鉴定了一个与水稻jacjacin相关的甘露糖结合凝集素基因OsJRL。我们分析了OsJRL在不同胁迫条件下的表达规律。此外，我们在大肠杆菌和水稻中过表达OsJRL。OsJRL cDNA包含一个438bp的开放阅读框，编码145个氨基酸的多肽。OsJRL定位于细胞核和细胞质。Real - time PCR分析显示，水稻中OsJRL的表达具有组织特异性，在盐、干旱、冷、热和脱落酸等不同胁迫下表达上调。在大肠杆菌中过表达OsJRL可增强细胞活力，显著提高高盐耐受性。水稻中OsJRL的过表达还增强了耐盐性，并增加了多个胁迫相关基因的表达水平，包括3个LEA（晚期胚胎发育丰富蛋白）基因（OsLEA19a、OsLEA23和OsLEA24）、3个Na（+）转运蛋白基因（OsHKT1;3、OsHKT1；4和OsHKT1；5）和2个DREB基因（OsDREB1A和OsDREB2B）。基于这些结果，我们认为OsJRL在细胞保护和应激信号转导中起重要作用。
Salinity, which is one of the most common abiotic stresses, may severely affect plant productivity and quality. Although plant lectins are thought to play important roles in plant defense signaling during pathogen attack, little is known about the contribution of plant lectins to stress resistance.We cloned and functionally characterized a rice jacalin-related mannose-binding lectin gene, OsJRL, from rice 'Nipponbare'. We analyzed the expression patterns of OsJRL under various stress conditions in rice. Furthermore, we overexpressed OsJRL in Escherichia coli and rice.The cDNA of OsJRL contained a 438bp open reading frame, which encodes a polypeptide of 145 amino acids. OsJRL was localized in the nucleus and cytoplasm. Real time PCR analyses revealed that OsJRL expression showed tissue specificity in rice and was upregulated under diverse stresses, namely salt, drought, cold, heat and abscisic acid treatments. Overexpression of OsJRL in E.coli enhanced cell viability and dramatically improved tolerance of high salinity. Overexpression of OsJRL in rice also enhanced salinity tolerance and increased the expression levels of a number of stress-related genes, including three LEA (late embryogenesis abundant proteins) genes (OsLEA19a, OsLEA23 and OsLEA24), three Na(+)transporter genes (OsHKT1;3, OsHKT1;4 and OsHKT1;5) and two DREB genes (OsDREB1A and OsDREB2B).Based on these results, we suggest that OsJRL plays an important role in cell protection and stress signal transduction.