Dinitroaniline herbicide resistance in a multiple-resistant Lolium rigidum population
Dinitroaniline herbicide resistance in a multiple-resistant Lolium rigidum population
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DOI：
10.1002/ps.4790
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发表时间：
2018-04-01
影响因子：
4.1
通讯作者：
Powles, Stephen
中科院分区：
文献类型：
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作者：
Chen, Jinyi;Yu, Qin;Powles, Stephen
出苗前的二硝基苯胺除草剂（如氟乐灵和二甲戊灵）对澳大利亚的免耕农业系统至关重要。从西澳谷物带收集的硬直黑麦草人口12年氟乐灵使用历史的特点是耐二硝基苯胺，乙酰辅酶A羧化酶（ACCase）和乙酰乳酸合成酶（ALS）抑制除草剂。对靶点抗性机制进行了研究。硬实种群对氟乐灵的抗性是敏感种群的32倍。它还显示出12至30倍的交叉抗性，其他二硝基苯胺除草剂（二甲戊乐灵，乙氟乐灵和安磺灵）。此外，该种群对常用的芽后ACCase和ALS抑制除草剂表现出多重抗性。在其它抗二硝基苯胺杂草中发现了2个微管蛋白靶位点突变（瓦尔-202-Phe和Thr-239-Ile），在同一种群中发现了ACC酶（Ile-1781-Leu，Asp-2078-Gly和Cys-2088-Arg）和ALS（Pro-197-Gln/Ser）的靶位点突变。建立了一种基于琼脂的培养皿筛选方法，用于二硝基苯胺类除草剂抗性的快速诊断。刚性种群本文首次报道了L.刚性，可能是负责二硝基苯胺耐药在这个群体。早期检测二硝基苯胺类除草剂的抗性和综合杂草管理策略，需要保持二硝基苯胺类除草剂的有效性。(c)2017年化学工业协会
BACKGROUNDThe pre-emergence dinitroaniline herbicides (such as trifluralin and pendimethalin) are vital to Australian no-till farming systems. A Lolium rigidum population collected from the Western Australian grain belt with a 12-year trifluralin use history was characterised for resistance to dinitroaniline, acetyl CoA carboxylase (ACCase)- and acetolactate synthase (ALS)-inhibiting herbicides. Target-site resistance mechanisms were investigated.RESULTSThis L. rigidum population exhibited 32-fold resistance to trifluralin, as compared with the susceptible population. It also displayed 12- to 30-fold cross-resistance to other dinitroaniline herbicides (pendimethalin, ethalfluralin and oryzalin). In addition, this population showed multiple resistance to commonly used post-emergence ACCase- and ALS-inhibiting herbicides. Two target-site -tubulin gene mutations (Val-202-Phe and Thr-239-Ile) previously documented in other dinitroaniline-resistant weed species were identified, and some known target-site mutations in ACCase (Ile-1781-Leu, Asp-2078-Gly and Cys-2088-Arg) and ALS (Pro-197-Gln/Ser) were found in the same population. An agar-based Petri dish screening method was established for the rapid diagnosis of resistance to dinitroaniline herbicides.CONCLUSIONEvolution of target-site resistance to both pre- and post-emergence herbicides was confirmed in a single L. rigidum population. The -tubulin mutations Val-202-Phe and Thr-239-Ile, documented here for the first time in L. rigidum, are likely to be responsible for dinitroaniline resistance in this population. Early detection of dinitroaniline herbicide resistance and integrated weed management strategies are needed to maintain the effectiveness of dinitroaniline herbicides. (c) 2017 Society of Chemical Industry