Comparative analysis of chloroplast DNA in Pyrus species:: physical map and gene localization
Comparative analysis of chloroplast DNA in Pyrus species:: physical map and gene localization
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DOI：
10.1007/s00122-002-1003-4
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发表时间：
2003-01-01
影响因子：
5.4
通讯作者：
Uematsu, C
中科院分区：
文献类型：
--
作者：
Katayama, H;Uematsu, C
梨（Pyrus ussuriensis var. hondoensis（Nakai et菊池）peptide]使用5种限制性内切酶SalI、XhoI、BamHI、SacI和Pst I构建。这些信息将使研究梨属物种之间的系统发育关系成为可能。梨cpDNA是一个环状分子，总长度约为156kb，其中24.8kb的反向重复序列将其分为17kb和90kb两个单拷贝区。物理图谱中的核酸内切酶识别位点通过覆盖梨cpDNA的整个序列的13个λ噬菌体克隆的单消化和双消化来确定。29个基因定位在梨的cpDNA物理图谱上。梨cpDNA的结构在基因组大小和基因顺序上与烟草cpDNA基本相同。对梨属5个种（Pyrus pyrifolia、Pyrus ussuriensis、Pyrus calleryana、Pyrus elaeagrifolia和Pyrus communis）的cpDNA进行了RFLP分析。仅在梨品种的cpDNA中发现了两个突变，即一个缺失位点突变和一个长度突变（缺失）。这些突变定位在梨cpDNA的物理图谱上。与其他被子植物相比，梨属植物的cpDNA突变数量较少，表明梨属植物基因组高度保守。
A physical map of chloroplast DNA (cpDNA) of pear [Pyrus ussuriensis var. hondoensis (Nakai et Kikuchi) Rehder] was constructed using five restriction enzymes, SalI, XhoI, BamHI, SacI and PstI. This information will make it possible to investigate the phylogenetic relationships between Pyrus species. Pear cpDNA was found to be a circular molecule with a total size of about 156 kb in which two inverted repeats of 24.8 kb divide the molecule into small (17 kb) and large (90 kb) single-copy regions. The endonuclease recognition sites in the physical map were determined by single and double digestion of 13 lambda phage clones which covered the entire sequence of the pear cpDNA. Twenty nine genes were localized on the physical map of the pear cpDNA. The structure of pear cpDNA was almost the same in terms of genome size and gene order as that of tobacco cpDNA. RFLP analysis was carried out on cpDNAs from five Pyrus species (Pyrus pyrifolia, Pyrus ussuriensis, Pyrus calleryana, Pyrus elaeagrifolia and Pyrus communis). Two mutations, a recognition-site mutation and a length mutation (deletion), were found only in the cpDNA of P pyrifolia cultivars. These mutations were localized on the physical map of pear cpDNA. The number of mutations of cpDNA in Pyrus species are small in comparison with those of other angiosperms, suggesting a high degree of genome conservatism in Pyrus species.