Targeted gene delivery to skin cells in vivo: a comparative study of liposomes and polymers as delivery vehicles.
Targeted gene delivery to skin cells in vivo: a comparative study of liposomes and polymers as delivery vehicles.
复制标题
体内靶向基因递送至皮肤细胞：脂质体和聚合物作为递送载体的比较研究。
DOI：
10.1002/jps.10061
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发表时间：
2002
影响因子：
3.8
通讯作者：
Fahl,WilliamE
中科院分区：
文献类型：
--
作者：
Raghavachari,Nalini;Fahl,WilliamE
脂质体是微观的脂膜囊泡，为生物活性分子的局部、真皮递送提供了当前的策略。它们已成功用于将各种低分子量和高分子量分子递送到皮肤中，并且作为病毒介导的递送系统的替代品，开辟了皮肤基因治疗领域。本研究在 6 日龄幼鼠身上进行，以确定几种脂质体和非脂质体制剂的体内功效，包括磷脂脂质体及其阳离子或聚乙二醇化变体、非离子脂质体及其阳离子变体、PINC 聚合物（保护性、相互作用、非缩合聚合物）以及丙二醇：酒精：水混合物（米诺地尔的递送载体）在递送 β-半乳糖苷酶和荧光素酶报告基因进入皮肤细胞。根据我们对皮肤细胞中 β-半乳糖苷酶和荧光素酶报告基因表达的观察，我们在此报告非离子脂质体是最有效的透皮递送载体，其次是非离子/阳离子和磷脂（聚乙二醇化）脂质体。丙二醇：乙醇：水混合物和 PINC 聚合物在 β-半乳糖苷酶或荧光素酶 DNA 的递送方面相对效率较低。这种使用非离子脂质体递送生物分子的简单、非侵入性技术为基因治疗和药物递送至真皮器官部位提供了有效的递送策略。 © 2002 Wiley-Liss, Inc. 和美国制药协会 J Pharm Sci 91:615–622, 2002
Liposomes are microscopic lipid membrane vesicles that provide a current strategy for topical, dermal delivery of biologically active molecules. They have been successfully used for the delivery of various low and high molecular weight molecules into the skin, and as an alternative to virus-mediated delivery systems, have opened the field of dermal gene therapy. The present study was undertaken on 6-day-old rat pups to determinein vivothe efficacy of several liposome and nonliposome formulations, including phospholipid liposomes and their cationic or pegylated variants, nonionic liposomes and their cationic variant, PINC polymer (Protective,Interactive,Noncondensing polymers), and a propylene glycol:alcohol:water mixture (delivery vehicle for minoxidil) in delivering β-galactosidase and luciferase reporter genes into skin cells. Based upon our observations of the expression of β-galactosidase and luciferase reporter genes in skin cells, we report here that nonionic liposomes are the most efficient vehicle for transdermal delivery followed by nonionic/cationic and phospholipid (pegylated) liposomes. The propylene glycol:ethanol:water mixture and the PINC polymer were relatively inefficient in the delivery of β-galactosidase or luciferase DNAs. This simple, noninvasive technique of using nonionic liposomes to deliver biomolecules provides an efficient delivery strategy for gene therapy and drug delivery to the dermal organ site. © 2002 Wiley-Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 91:615–622, 2002