INTRAGLOMERULAR T-CELLS AND MONOCYTES IN NEPHRITIS - STUDY WITH MONOCLONAL-ANTIBODIES
INTRAGLOMERULAR T-CELLS AND MONOCYTES IN NEPHRITIS - STUDY WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1038/ki.1987.123
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发表时间：
1987-05-01
影响因子：
19.6
通讯作者：
REUBEN, R
中科院分区：
文献类型：
--
作者：
NOLASCO, FEB;CAMERON, JS;REUBEN, R
利用单克隆抗体和免疫过氧化物酶技术，在肾小球肾炎患者的肾活检中寻找肾小球内T细胞、单核细胞、总白细胞和其他单核亚群。我们研究了24例光学显微镜下无明显肾小球增生的活检，32例只有毛细血管内细胞增多，21例有毛细血管外月牙状肾小球疾病。非增生性组肾小球内白细胞较少。相反，与该组相比，肾小球高细胞性活检，特别是月牙组的活检显示肾小球内总白细胞和单核/巨噬细胞数量增加，T淋巴细胞和T细胞毒性/抑制细胞过量，只有月牙组的T辅助/诱导淋巴细胞显著增加。各组均有少量B淋巴细胞和NK细胞。月牙组肾小球总t细胞数和每肾小球横截面单核细胞数高度相关。与非增生性组相比，只有特发性IgA肾病未能显示肾小球内白细胞数量的显著增加，相反，henoche - schoenlein紫癜活检显示单核细胞和T细胞亚群均过量。增殖性肾炎肾小球中T淋巴细胞和单核细胞的发现表明细胞免疫机制可能在其发病机制中起作用，特别是当新月存在时。
Intraglomerular T cells, monocytes, total leucocytes and other mononuclear subsets were sought in renal biopsies from patients with glomerulonephritis, using monoclonal antibodies and immunoperoxidase techniques. Twenty-four biopsies with no significant glomerular proliferation on optical microscopy, thirty-two with only endocapillary hypercellularity, and twenty-one with extra capillary crescentic glomerular disease were studied. Few intra-glomerular leucocytes were seen in the non-proliferative group. In contrast, when compared with this group, biopsies with glomerular hypercellularity and particularly those with crescents showed increased numbers of intra-glomerular total leucocytes and monocytes/macrophages, as well as an excess of T lymphocytes and T cytotoxic/suppressor cells: T helpr/inducer lymphocytes were significantly increased only in the crescentic group. Only small numbers of B lymphocytes and NK cells were found in all groups. The numbers of total glomerular T-cells and monocytes per glomerular cross section were highly correlated in the crescentic group. Only idiopathic IgA nephropathy failed to show a significant increase in the numbers of intra-glomerular leucocytes, in comparison with the nonproliferative group, Henoch-Schoenlein purpura biopsies in contrast had an excess of both monocytes and T cell subsets. The finding of T lymphocytes as well as monocytes in glomeruli of proliferative nephritis suggests that cellular immune mechanisms may play a role in their pathogenesis, especially when crescents are present.