Effect of surface modification of rat erythrocytes of different ages on their partitioning behavior in charge-sensitive two-polymer aqueous phases.
Effect of surface modification of rat erythrocytes of different ages on their partitioning behavior in charge-sensitive two-polymer aqueous phases.
复制标题
不同年龄大鼠红细胞表面修饰对其在电荷敏感双聚合物水相中分配行为的影响。
DOI：
10.1016/0005-2736(86)90565-1
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Seaman,GV
中科院分区：
文献类型：
--
作者：
Walter,H;Krob,EJ;Pedram,A;Tamblyn,CH;Seaman,GV
两种聚合物水相系统中细胞之间的分配差异源于细胞表面性质之间的细微差异。由于影响分配比（P）的参数与P本身之间的指数关系，怀疑影响密切相关的细胞群体的差异分配的膜组分的差异不能通过常规化学测定技术直接建立。为了研究负责大鼠红细胞表面性质的年龄相关变化的组分的化学性质，我们设计了一种方法，该方法使用不同细胞年龄的红细胞亚群的同位素标记与不同的酶和/或化学处理的组合，然后在电荷敏感的两种聚合物水相系统中逆流分布。这些研究表明：（a）神经氨酸酶敏感性唾液酸不是通过分配检测到的细胞年龄相关表面差异的原因;（B）可以用乙醇（从经酶解固定的红细胞）提取或用稀硫酸裂解引起细胞年龄相关表面差异的组分。我们的数据是一致的假设，神经节苷脂连接的唾液酸是负责不同年龄的大鼠血细胞之间的细胞电荷相关的表面差异的化学部分。
Partitioning differences between cells in two-polymer aqueous phase systems originate from subtle differences between the surface properties of cells. Because of the exponential relation between the parameters affecting the partition ratio (P) and thePitself, differences in membrane components suspected of affecting the differential partitioning of closely related cell populations cannot be directly established by conventional chemical assay techniques. In order to study the chemical nature of the components responsible for the age-related changes in surface properties of rat red cells we have devised an approach which uses a combination of isotopic labeling of erythrocyte subpopulations of distinct cell age with different enzyme and/or chemical treatments followed by countercurrent distribution in charge-sensitive two-polymer aqueous phase systems. These studies show that: (a) neuraminidase-susceptible sialic acid is not responsible for the cell age-related surface differences detected by partitioning; (b) the component(s) responsible for the cell age-related surface differences can be extracted (from aldehyde-fixed red cells) with ethanol or cleaved with dilute sulfuric acid. Our data are consistent with the hypothesis that ganglioside-linked sialic acid is the chemical moiety responsible for the cell charge-associated surface differences among rat blood cells of different ages.