A new experimental model of glaucoma in rats through intracameral injections of hyaluronic acid
A new experimental model of glaucoma in rats through intracameral injections of hyaluronic acid
复制标题
DOI：
10.1016/j.exer.2005.01.008
复制
发表时间：
2005-07-01
影响因子：
3.4
通讯作者：
Rosenstein, RE
中科院分区：
文献类型：
--
作者：
Moreno, MC;Marcos, HJA;Rosenstein, RE
压力性视神经损伤的实验模型将有助于理解导致神经节细胞死亡的细胞事件，以及它们如何受到眼压和其他与青光眼相关的危险因素的影响。本报告的目的是研究玻璃质酸引起的大鼠长期眼压升高对视网膜电活动和视网膜及视神经组织学的影响。为此，每周在大鼠一只眼的前房内注射透明质酸，而对侧眼注射生理盐水。结果表明，透明质酸给药3周或6周后，暗适应视网膜电流图的振荡电位和a、b波波幅均显著降低。透明质酸治疗10周后，这些参数进一步降低。与注射生理盐水的动物相比，注射透明质酸10周的动物的前房角结构没有明显的变化，而注射透明质酸10周的动物的神经节细胞层细胞和视神经轴突显著丢失。综上所述，目前的结果表明，长期应用透明质酸可导致视网膜和视神经的视网膜电活动和组织学改变显著降低，这似乎与慢性开角型青光眼的某些特征相一致。因此，这可能是一个研究高眼压损害视神经和视网膜的细胞机制的实验模型。(C)2005爱思唯尔有限公司。保留所有权利。
An experimental model of pressure-induced optic nerve damage would greatly facilitate the understanding of the cellular events leading to Ganglion cell death, and how they are influenced by intraocular pressure and other risk factors associated to glaucoma. The aim of the present report was to study the effect of a long-term increase of intraocular pressure in rats induced by intracameral injections of hyaluronic acid with respect to electroretinographic activity and retinal and optic nerve histology. For this purpose, hyaluronic acid was injected weekly in the rat anterior chamber of one eye, whereas the contralateral eye was injected with saline solution. The results showed a significant decrease of oscillatory potentials and a- and b-wave amplitude of the scotopic electroretinogram after 3 or 6 weeks of hyaluronic acid administration, respectively. These parameters were further reduced after 10 weeks of treatment with hyaluronic acid. No significant changes in anterior chamber angle structures from hyaluronic acid- and vehicle-injected eyes were observed, whereas a significant loss of ganglion cell layer cells and of optic nerve axons were detected in animals that received hyaluronic acid for 10 weeks, as compared to eyes injected with saline solution. In summary, present results indicate that the chronic administration of hyaluronic acid induced a significant decrease in the electroretinographic activity and histological changes in the retina and optic nerve that seem consistent with some features of chronic open-angle glaucoma. Therefore, this could be an experimental model to study the cellular mechanisms by which elevated intraocular pressure damages the optic nerve and the retina. (c) 2005 Elsevier Ltd. All rights reserved.