REACTIVE LYSIS - COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS .2. CHARACTERIZATION OF ACTIVATED REACTOR AS C56BAR AND PARTICIPATION OF C8 AND C9
REACTIVE LYSIS - COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS .2. CHARACTERIZATION OF ACTIVATED REACTOR AS C56BAR AND PARTICIPATION OF C8 AND C9
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DOI：
10.1084/jem.131.4.643
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发表时间：
1970-01-01
影响因子：
15.3
通讯作者：
THOMPSON, RA
中科院分区：
文献类型：
--
作者：
LACHMANN, PJ;THOMPSON, RA
材料和方法这些在前一篇论文(1)中有描述。氟磷酸二异丙基（DFP）来自英国诺丁汉Boots Pure Drug Co.， 1:10邻菲罗啉水合物来自英国埃塞克斯郡Chadwell Heath的Hopkins & Williams， Ltd.。在二甲基甲酰胺中配制0.5~溶液，在E/EDTA/琼脂糖混合物中以1:50稀释。1活化反应器的定量。活化反应器的估计是通过在E/EDTA/琼脂糖板中填充一个标准尺寸的孔，并在室温下扩散18小时来获得的。然后将板浸入1:50的豚鼠EDTA补体中，37℃孵育6小时。测定了裂解区直径。
Materials and MethodsThese are described in the preceding paper (1). Diisopropyl fluorophosphate (DFP) was obtained from Boots Pure Drug Co., Nottingham, England. 1: 10 phenanthroline hydrate was obtained from Hopkins & Williams, Ltd., Chadwell Heath, Essex, England. A 0.5~ solution was made in dimethylformamide and this was diluted 1: 50 in the E/EDTA/agarose mixture. 1 Quantitation of Activated Reactor.--An estimate of activated reactor was obtained by filling a standard size well in an E/EDTA/agarose plate with activated reactor and allowing diffusion to occur for 18 hr at room temperature. The plate was then immersed in 1: 50 guinea pig EDTA complement and incubated at 37 C for 6 hr. The diameter of the zone of lysis was measured.