Expression of nonmuscle myosin heavy chain-B isoform in the vessel wall of porcine coronary arteries after balloon angioplasty.
Expression of nonmuscle myosin heavy chain-B isoform in the vessel wall of porcine coronary arteries after balloon angioplasty.
复制标题
球囊血管成形术后猪冠状动脉血管壁中非肌肉肌球蛋白重链 B 亚型的表达。
DOI：
10.1161/01.res.80.4.514
复制
发表时间：
1997
影响因子：
20.1
通讯作者：
Wilcox,JN
中科院分区：
文献类型：
--
作者：
DeLeon,H;Scott,NA;Martin,F;Simonet,L;Bernstein,KE;Wilcox,JN
非肌球蛋白重链b异构体（NMMHC-B）通过增殖的血管平滑肌细胞（SMCs）表达，其在原发性病变中的表达被认为可以预测动脉粥样硬化切除术后再狭窄的发生。本研究采用原位杂交和免疫组织化学方法研究猪冠状动脉血管成形术后NMMHC-B的时间过程表达。国产幼猪采用标准临床血管成形术导管对左前降支和旋支冠状动脉进行经皮腔内冠状动脉成形术（PTCA）。为了鉴定增殖细胞，给药5 ' -溴-2 ' -脱氧尿苷（BrdU），并在连续切片上进行免疫组织化学检测。在球囊血管成形术后3、7和14天检查血管，并以未损伤的冠状动脉血管作为对照。正常动脉与35s标记的主要位于内层的NMMHC-B核探针杂交。球囊成形术后3 d外膜内NMMHC-B表达明显升高。损伤后7天和14天，含NMMHC-B mrna的细胞定位于动脉损伤部位的外膜和新生内膜。BrdU染色显示，血管成形术后3天和7天，细胞增殖与NMMHC-B mRNA表达共定位。这些数据表明外膜和新生内膜中增殖的细胞表达NMMHC-B；然而，它的表达并不局限于增殖状态，因为NMMHC-B mRNA也存在于正常冠状动脉的静止SMCs中，以及血管成形术后14天的非增殖的上皮细胞和新生内膜细胞中。
Nonmuscle myosin heavy chain-B isoform (NMMHC-B) is expressed by proliferating vascular smooth muscle cells (SMCs), and its expression in primary lesions has been proposed to be predictive of restenosis after atherectomy. The present study was designed to study the time-course expression of NMMHC-B after angioplasty of porcine coronary arteries by in situ hybridization and immunohistochemistry. Domestic juvenile swine underwent percutaneous transluminal coronary angioplasty (PTCA) of the left anterior descending and circumflex coronary arteries with standard clinical angioplasty catheters. To identify proliferating cells, 5′-bromo-2′-deoxyuridine (BrdU) was administered and detected by immunohistochemistry on serial sections. Vessels were examined at 3, 7, and 14 days after balloon angioplasty, and uninjured coronary vessels were used as controls. Normal arteries showed hybridization to35S-labeled NMMHC-B riboprobes localized mainly in the medial layer. NMMHC-B expression in the adventitia was markedly increased 3 days after balloon angioplasty. Seven and 14 days after injury, NMMHC-B mRNA–containing cells were localized in the adventitia and neointima at the arterial injury site. Cell proliferation, as indicated by BrdU staining, colocalized with NMMHC-B mRNA expression 3 and 7 days after angioplasty. These data indicate that cells proliferating in the adventitia and neointima express NMMHC-B; however, its expression is not limited to the proliferative state, since NMMHC-B mRNA was also found in quiescent SMCs of normal coronary arteries and in nonproliferating adventitial and neointimal cells 14 days after angioplasty.