Localization of the human angiogenin gene to chromosome band 14q11, proximal to the T cell receptor alpha/delta locus.
Localization of the human angiogenin gene to chromosome band 14q11, proximal to the T cell receptor alpha/delta locus.
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人类血管生成素基因定位于染色体带 14q11，靠近 T 细胞受体 α/δ 基因座。
DOI：
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发表时间：
1990
影响因子：
9.8
通讯作者：
B. Vallée
中科院分区：
文献类型：
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作者：
S. Weremowicz;E. Fox;C. Morton;B. Vallée
编码血管生成素的基因是一种有效的血管形成诱导剂，已定位于人类基因组中。根据对人-啮齿动物杂交细胞系的不一致性分析，它以每个单倍体基因组的单拷贝形式存在，并定位在14号染色体上。通过将血管生成素探针与正常人淋巴细胞和RPMI8402细胞的中期染色体进行原位杂交，这种定位被细化到14q11。来自RPMI8402细胞的结果还证实，血管生成素基因位于T细胞受体α/增量基因座内易位断裂点的近端，因此位于该基因座的上游。在人类胎盘DNA的编码区发现了一个频率为29%的AvaII RFLP，它是由一次沉默的颠换引起的。
The gene encoding angiogenin, a potent inducer of blood vessel formation, has been localized within the human genome. It is present as a single copy per haploid genome and is located on chromosome 14, on the basis of discordancy analysis of human-rodent hybrid cell lines. This localization was refined to 14q11 by in situ hybridization of an angiogenin probe to metaphase chromosomes prepared from both normal human lymphocytes and RPMI 8402 cells. The results from the RPMI 8402 cells also establish that the angiogenin gene resides proximal to a translocation breakpoint within the T cell receptor alpha/delta locus and therefore upstream from that locus. An AvaII RFLP, present at a frequency of 29% in an unselected collection of human placental DNAs, was identified in the coding region of the gene and results from a single silent transversion.