DEGENERATED HUMAN INTERVERTEBRAL DISCS CONTAIN AUTOANTIBODIES AGAINST EXTRACELLULAR MATRIX PROTEINS
DEGENERATED HUMAN INTERVERTEBRAL DISCS CONTAIN AUTOANTIBODIES AGAINST EXTRACELLULAR MATRIX PROTEINS
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DOI：
10.22203/ecm.v027a18
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发表时间：
2014-01-01
影响因子：
3.1
通讯作者：
Stoyanov, J. V.
中科院分区：
文献类型：
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作者：
Capossela, S.;Schlafli, P.;Stoyanov, J. V.
腰椎间盘退变与背部疼痛和炎症细胞水平升高有关。有假说认为，间盘源性疼痛是血管和神经沿着环裂生长的直接结果，这可能会使无血管髓核暴露在全身循环中，并引发自身免疫反应。在这项研究中，我们证实了我们之前在体外培养的人类退行性和创伤后IVD中观察到的抗体。我们假设抗体的存在是由于对椎间盘特定蛋白质的自身免疫反应。此外，我们还确定了在退行性腰椎间盘疾病中可能触发自身免疫反应的抗原。我们证明退行性和创伤后的IVD含有针对典型的椎间盘细胞外蛋白的抗体，特别是NP的蛋白。我们鉴定了针对II型胶原和聚集素的免疫球蛋白，证实了针对正常免疫特权NP的自身免疫反应。我们还发现了针对I型和V型胶原的特异性免疫球蛋白，但不针对III型胶原。综上所述，本研究证实了椎间盘退变与自身免疫之间的关系，并可能为进一步开发退行性腰椎间盘疾病的预后、诊断和治疗监测标记物开辟道路。
Degeneration of intervertebral discs (IVDs) is associated with back pain and elevated levels of inflammatory cells. It has been hypothesised that discogenic pain is a direct result of vascular and neural ingrowth along annulus fissures, which may expose the avascular nucleus pulposus (NP) to the systemic circulation and induce an autoimmune reaction. In this study, we confirmed our previous observation of antibodies in human degenerated and posttraumatic IVDs cultured in vitro. We hypothesised that the presence of antibodies was due to an autoimmune reaction against specific proteins of the disc. Furthermore we identified antigens which possibly trigger an autoimmune response in degenerative disc diseases. We demonstrated that degenerated and post-traumatic IVDs contain IgG antibodies against typical extracellular proteins of the disc, particularly proteins of the NP. We identified IgGs against collagen type II and aggrecan, confirming an autoimmune reaction against the normally immune privileged NP. We also found specific IgGs against collagens types I and V, but not against collagen type III. In conclusion, this study confirmed the association between disc degeneration and autoimmunity, and may open the avenue for future studies on developing prognostic, diagnostic and therapymonitoring markers for degenerative disc diseases.