Convergence of TOR-nitrogen and Snf1-glucose signaling pathways onto Gln3
Convergence of TOR-nitrogen and Snf1-glucose signaling pathways onto Gln3
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DOI：
10.1128/mcb.22.4.1246-1252.2002
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发表时间：
2002-02-01
影响因子：
5.3
通讯作者：
Zheng, XFS
中科院分区：
文献类型：
--
作者：
Bertram, PG;Choi, JH;Zheng, XFS
碳和氮是细胞生物的两种基本营养源。它们为能量代谢和代谢生物合成提供前体。在酿酒酵母中，已经描述了不同的传感和信号传导途径，其分别响应于碳源和氮源的质量来调节基因表达。Gln3是氮代谢产物抑制基因（NCR）的GATA型转录因子。先前的观察表明，氮源的质量通过雷帕霉素（TOR）蛋白的靶标控制Gln3的磷酸化和细胞质保留。在这项研究中，我们表明葡萄糖还调节Gln 3磷酸化和亚细胞定位，这是由Snf 1（AMP依赖性蛋白激酶的酵母同源物和细胞质葡萄糖传感器）介导的。我们的数据表明，葡萄糖和氮信号通路收敛到Gln3，这可能是营养传感和饥饿反应的关键。
Carbon and nitrogen are two basic nutrient sources for cellular organisms. They supply precursors for energy metabolism and metabolic biosynthesis. In the yeast Saccharomyces cerevisiae, distinct sensing and signaling pathways have been described that regulate gene expression in response to the quality of carbon and nitrogen sources, respectively. Gln3 is a GATA-type transcription factor of nitrogen catabolite-repressible (NCR) genes. Previous observations indicate that the quality of nitrogen sources controls the phosphorylation and cytoplasmic retention of Gln3 via the target of rapamycin (TOR) protein. In this study, we show that glucose also regulates Gln3 phosphorylation and subcellular localization, which is mediated by Snf1, the yeast homolog of AMP-dependent protein kinase and a cytoplasmic glucose sensor. Our data show that glucose and nitrogen signaling pathways converge onto Gln3, which may be critical for both nutrient sensing and starvation responses.