Double-time is a novel Drosophila clock gene that regulates PERIOD protein accumulation
Double-time is a novel Drosophila clock gene that regulates PERIOD protein accumulation
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DOI：
10.1016/s0092-8674(00)81224-6
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发表时间：
1998-07-10
期刊：
影响因子：
64.5
通讯作者：
Young, MW
中科院分区：
文献类型：
--
作者：
Price, JL;Blau, J;Young, MW
我们分离出了一个新的果蝇生物钟基因——doubletime（dbt）的三个等位基因。短周期（dbts）和长周期（dbt(L)）突变体在行为节律和分子振荡方面均不同于先前已确定的生物钟基因period和timeless。第三个等位基因dbf(P)导致蛹死亡，并消除幼虫中per和tim基因产物的昼夜节律循环。在dbf(P)突变体中，PER蛋白持续积累，保持低磷酸化状态，并且其积累不再依赖于TIM蛋白。我们提出，DOUBLETIME蛋白的正常功能是降低单体PER蛋白的稳定性，从而降低其积累水平。这将促使per/tim转录与PER/TIM复合物功能之间产生延迟，这对分子节律至关重要。
We have isolated three alleles of a novel Drosophila clock gene, double-time (dbt). Short- (dbts) and long-period (dbt(L)) mutants after both behavioral rhythmicity and molecular oscillations from previously identified clock genes, period and timeless. A third allele, dbf(P), causes pupal lethality and eliminates circadian cycling of per and tim gene products in larvae. In dbt(P) mutants, PER proteins constitutively accumulate, remain hypophosphorylated, and no longer depend on TIM proteins for their accumulation. We propose that the normal function of DOUBLETIME protein is to reduce the stability and thus the level of accumulation of monomeric PER proteins. This would promote a delay between per/tim transcription and PER/TIM complex function, which is essential for molecular rhythmicity.