Surface antigens of Biomphalaria glabrata (Gastropoda) hemocytes: occurrence of membrane-associated hemolymph-like factors antigenically related to snail hemoglobin.
Surface antigens of Biomphalaria glabrata (Gastropoda) hemocytes: occurrence of membrane-associated hemolymph-like factors antigenically related to snail hemoglobin.
复制标题
光滑双脐动物（腹足纲）血细胞的表面抗原：与蜗牛血红蛋白抗原相关的膜相关血淋巴样因子的出现。
DOI：
10.1016/0022-2011(83)90248-3
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发表时间：
1983
影响因子：
3.4
通讯作者：
Yoshino,TP
中科院分区：
文献类型：
--
作者：
Yoshino,TP
A polyvalent antiserum (anti-HPR) generated in rabbits to cell-free hemolymph from a PR albino (M-line) stock of snail,Biomphalaria glabrata, was employed as a membrane probe to determine if antigens related to snail hemolymph were associated with the surface membranes of phosphate-buffered saline (PBS) washed hemocytes from a schistosome-susceptible (PR albino) and refractory (10-R2) stock ofB. glabrata. Immunofluorescent and immunoelectron microscopical analyses revealed a strong cross-reactivity between anti-HPRantibodies and hemocytes from both PR albino and 10-R2 snails indicating the presence of surface-associated hemolymph or hemolymph-like antigens. Hemoglobin isolated from PR albinoB. glabratahemolymph competitively inhibited the binding of anti-HPRto hemocytes suggesting that cross-reactive membrane components were, at least in part, antigenically related to snail hemoglobin. Antigens reactive with antihemolymph antibodies also were resistant to protease treatment. No antigenic differences between PR albino and 10-R2 snail hemocytes could be detected due to the heterospecific nature of the probe antiserum, however, it is believed that the major cross-reactive membrane components, e.g., hemoglobin-like determinants, are shared in common by hemocytes of both snail stocks.
