Pro- and anti-inflammatory cytokine gene expression in rat sciatic nerve chronic constriction injury model of neuropathic pain
Pro- and anti-inflammatory cytokine gene expression in rat sciatic nerve chronic constriction injury model of neuropathic pain
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DOI：
10.1006/exnr.2001.7677
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发表时间：
2001-06-01
影响因子：
5.3
通讯作者：
Low, PA
中科院分区：
文献类型：
--
作者：
Okamoto, K;Martin, DP;Low, PA
细胞因子可能在病理生理上参与痛觉过敏，关于细胞因子的类型和它们的作用部位存在不确定性。为了研究关键的促炎和抗炎细胞因子在神经性疼痛的慢性收缩模型中的作用，使用竞争性RT-PCR定量TNF、IL-1 β、IL-6和IL-10的mRNA表达。在慢性压迫性损伤（CCI）后3、7、14、45天检测大鼠坐骨神经中各种细胞因子mRNA的表达，并进行行为学测试。与假手术组相比，CCI组在第3、7和14天的热温热和触摸阈值显著降低。坐骨神经中IL-1 β和IL-6的细胞因子基因表达在第7天显著增加，TNF在第14天显著增加。IL-10的表达经历逐渐和进行性增加，在第45天达到统计学显著性。(C)北京：科学出版社.
Cytokines may be pathophysiologically involved in hyperalgesia, Uncertainty exists about the types of cytokines and their site of action. To study the role of key pro- and anti-inflammatory cytokines in a chronic constriction model of neuropathic pain, mRNA expression of TNF, IL-1 beta, IL-6, and IL-10 was quantified using competitive RT-PCR. Each cytokine mRNA in rat sciatic nerve was examined at days 3, 7, 14, and 45 after chronic constriction injury (CCI), We also undertook behavioral testing of these rats. Thermal warming and touch thresholds were significantly reduced at days 3, 7, and 14 in the CCI group, compared with the sham-operated group. Cytokine gene expression in sciatic nerve was significantly increased at day 7 for IL-1 beta and IL-6 and at day 14 for TNF. Expression of IL-10 underwent a gradual and progressive increase, reaching statistical significance at day 45. (C) 2001 Academic Press.