Induction of chromosome aberrations and cell killing in Syrian hamster fibroblasts by gamma-rays, X-rays and fast neutrons.
Induction of chromosome aberrations and cell killing in Syrian hamster fibroblasts by gamma-rays, X-rays and fast neutrons.
复制标题
伽马射线、X 射线和快中子诱导叙利亚仓鼠成纤维细胞染色体畸变和细胞杀伤。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
P. D. Holt
中科院分区：
文献类型：
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作者：
C. J. Roberts;P. D. Holt
用60Co伽马射线、250千伏X射线、15 MeV中子或9Be(d，n)10B反应产生的平均能量为2.1 MeV的中子照射BHK21 C13叙利亚仓鼠成纤维细胞，观察其染色体畸变率。这些细胞处于静止期，集中在细胞周期的G1期。在实验不确定度范围内，对60Co伽马射线和250千伏X射线的响应之间没有可检测到的差异。R.b.e。对于双着丝粒的产生，根据响应的“一击”分量，对于15 MeV的中子是(5+/-2)，对于2.1 MeV的中子是(12+/-5)。对于每一次辐射，没有双着丝粒、中心环或无着丝粒碎片的细胞比例图与同一实验中获得的静止相细胞的存活曲线密切对应。
Chromosome aberrations were scored in BHK21 C13 Syrian hamster fibroblasts, exposed to 60Co gamma-rays, 250 kV X-rays, 15 MeV neutrons or neutrons of mean energy 2.1 MeV produced from the 9Be(d,n)10B reaction. The cells were irradiated in stationary phase, where they are concentrated in the G1 phase of the cell cycle. Within experimental uncertainty there was no detectable difference between the responses to 60Co gamma-rays and to 250kV X-rays. The r.b.e. for the production of dicentrics, based on the 'one-hit' component of response, was (5 +/- 2) for the 15 MeV neutrons and (12 +/- 5) for the 2.1 MeV neutrons. For each radiation, a graph of the proportion of cells without a dicentric, centric ring or acentric fragment corresponded closely to the survival curve for stationary-phase cells obtained in the same experiment.