Typical demethylation events in cloned pig embryos - Clues on species-specific differences in epigenetic reprogramming of a cloned donor genome
Typical demethylation events in cloned pig embryos - Clues on species-specific differences in epigenetic reprogramming of a cloned donor genome
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DOI：
10.1074/jbc.m106516200
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发表时间：
2001-10-26
影响因子：
4.8
通讯作者：
Han, YM
中科院分区：
文献类型：
--
作者：
Kang, YK;Koo, DB;Han, YM
我们调查了正常受精或体细胞核移植产生的猪着床前胚胎是否发生了基因组去甲基化过程。用亚硫酸氢盐测序技术分析着丝粒卫星和Pre-1短散布元件(正弦)序列的甲基化状态。在克隆供体基因组的这些重复序列中，在卵裂过程中发现了典型的去甲基化过程，其模式与受精的供体基因组中检测到的类似。这些发现与之前对克隆牛胚胎的观察不同，在克隆牛胚胎中，供体基因组的不同重复区域显示出异常的甲基化模式。我们的结果表明，在改变克隆供体基因组的表观遗传状态方面存在物种特异性差异。
We investigated whether a genomic demethylation process occurs in pig preimplantation embryos produced by either normal fertilization or somatic cell nuclear transfer. The methylation status of the centromeric satellite and the PRE-1 short interspersed element (SINE) sequences was characterized using bisulfite-sequencing technology. Typical demethylation processes were identified in these repetitive sequences of the cloned donor genome during cleavage, the patterns of which were similar to the ones detected in fertilized counterparts. These findings are different from previous observations with cloned bovine embryos where various repeated regions of the donor genome exhibited aberrant methylation patterns. Our results indicate that species-specific differences exist in modifying the epigenetic status of cloned donor genomes.