High-Level Genetic Diversity but No Population Structure Inferred from Nuclear and Mitochondrial Markers of the Peritrichous Ciliate Carchesium polypinum in the Grand River Basin (North America)
High-Level Genetic Diversity but No Population Structure Inferred from Nuclear and Mitochondrial Markers of the Peritrichous Ciliate Carchesium polypinum in the Grand River Basin (North America)
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DOI：
10.1128/aem.00178-09
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发表时间：
2009-05-15
影响因子：
4.4
通讯作者：
Lynn, D. H.
中科院分区：
文献类型：
--
作者：
Gentekaki, E.;Lynn, D. H.
评估纤毛虫种内遗传变异的研究很少，而且是最近的。因此，对这一问题的了解和对这一问题背后的过程的理解是有限的。我们试图评估种内遗传变异的程度在Carchesium polypinum（纤毛门：周毛目），一个世界性的，淡水纤毛虫。我们分离了C.来自加拿大西南部安大略的格兰德河流域的位置的珊瑚虫。然后，我们利用核标记ITS 1、ITS 2和大亚基rRNA的高变区以及线粒体细胞色素c氧化酶I基因（考克斯-1）的819 bp片段来研究C.息肉和上述标记在群体水平上的分辨率程度。我们还试图确定该生物体是否表现出任何与该地区地理相关的种群结构。我们的研究表明，有一个高度的遗传多样性，在分离物水平上，揭示了线粒体标记，而不是核标记。此外，我们的研究结果表明，C。珊瑚虫可能不是以前认为的单一形态种。
Studies that assess intraspecific genetic variation in ciliates are few and quite recent. Consequently, knowledge of the subject and understanding of the processes that underlie it are limited. We sought to assess the degree of intraspecific genetic variation in Carchesium polypinum (Ciliophora: Peritrichia), a cosmopolitan, freshwater ciliate. We isolated colonies of C. polypinum from locations in the Grand River basin in Southwestern Ontario, Canada. We then used the nuclear markers-ITS1, ITS2, and the hypervariable regions of the large subunit rRNA-and an 819-bp fragment of the mitochondrial cytochrome c oxidase I gene (cox-1) to investigate the intraspecific genetic variation of C. polypinum and the degree of resolution of the above-mentioned markers at the population level. We also sought to determine whether the organism demonstrated any population structure that mapped onto the geography of the region. Our study shows that there is a high degree of genetic diversity at the isolate level, revealed by the mitochondrial markers but not the nuclear markers. Furthermore, our results indicate that C. polypinum is likely not a single morphospecies as previously thought.