EXPRESSION OF V-SRC AND CHICKEN C-SRC IN RAT-CELLS DEMONSTRATES QUALITATIVE DIFFERENCES BETWEEN PP60V-SRC AND PP60C-SRC
EXPRESSION OF V-SRC AND CHICKEN C-SRC IN RAT-CELLS DEMONSTRATES QUALITATIVE DIFFERENCES BETWEEN PP60V-SRC AND PP60C-SRC
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DOI：
10.1016/0092-8674(84)90308-8
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
BISHOP, JM
中科院分区：
文献类型：
--
作者：
PARKER, RC;VARMUS, HE;BISHOP, JM
逆转录病毒致癌基因v-src。这些基因之间的相似性提高了c-src可能引起肿瘤生长的可能性。构建了一个允许表达鸡c-src的嵌合质粒。在用嵌合DNA转染大鼠2细胞后，分离出含有10倍于正常细胞内水平的pp 60 c-src的大鼠细胞系。这些细胞产生c-src编码的蛋白质（pp 60 c-src），其量至少是v-src产物（pp 60 v-src）转化所需量的3倍。细胞保持表型正常，含有肌动蛋白电缆，不在软琼脂中生长。用v-src的分子克隆转染含有pp 60 c-src或Rat-2细胞的升高的细胞的细胞系产生表现出生物转化细胞的性质的细胞;圆形形态、断裂的肌动蛋白索和在软琼脂中生长的能力。
The retroviral oncogene v-src. The similarity between these genes raised the possibility that c-src might be able to elicit neoplastic growth. A chimeric plasmid was constructed that allows the expression of chicken c-src. A rat cell line containing 10 times the normal intracellular level of pp60c-src was isolated after transfecting rat-2 cells with the chimeric DNA. These cells produce the protein encoded by c-src (pp60c-src) in quantities at least 3 times greater than required to achieve transformation by the product of v-src (pp60v-src). The cells remain phenotypically normal, contain actin cables and do not grow in soft agar. Transfection of the cell line containing elevated cells of pp60c-src or Rat-2 cells with a molecular clone of v-src produces cells that exhibit properties of biologically transformed cells; round morphology, disrupted actin cables and ability to grow in soft agar.