Influence of host genetics in shaping the rumen bacterial community in beef cattle
Influence of host genetics in shaping the rumen bacterial community in beef cattle
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DOI：
10.1038/s41598-020-72011-9
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发表时间：
2020-09-15
影响因子：
4.6
通讯作者：
Fernando, Samodha C.
中科院分区：
文献类型：
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作者：
Abbas, Waseem;Howard, Jeremy T.;Fernando, Samodha C.
根据最近的宿主-微生物关联研究，正在形成一种共识，即胃肠道微生物区系的物种组成是由宿主遗传因素和环境之间的相互作用决定的多基因特征。在这里，我们通过进行全基因组关联研究，研究了宿主遗传因素对形成瘤胃细菌种类组成的影响。利用在牛基因组中发现的一组常见的61,974个单核苷酸多态(n=586)和相应的瘤胃细菌群落组成，我们确定了遗传力较高的操作分类单位(OTU)、科和门。顶端组合(1-Mb窗口)位于7条染色体上。这些区域以多种方式与瘤胃微生物区系相关联；一些(19号染色体；位置3.0-4.0Mb)与密切相关的分类群(Prevotalaceae、Paraprevotalaceae和RF16)相关，一些(27号染色体；位置3.0-4.0Mb)与远缘的分类群(Prevotalaceae、Fibrobacaceae、RF16、RFP12、S24-7、Lentisphaerae和Tenericuts)有关，而另一些(染色体23；位置0.0-1.0)与相关和无关的分类群有关。与已识别的基因组区域相关的注释基因表明，观察到的关联是为了选择性地吸收瘤胃中的挥发性脂肪酸，以增加宿主的能量利用率。本研究证实了宿主遗传因素对瘤胃细菌群落组成的影响。
In light of recent host-microbial association studies, a consensus is evolving that species composition of the gastrointestinal microbiota is a polygenic trait governed by interactions between host genetic factors and the environment. Here, we investigated the effect of host genetic factors in shaping the bacterial species composition in the rumen by performing a genome-wide association study. Using a common set of 61,974 single-nucleotide polymorphisms found in cattle genomes (n=586) and corresponding rumen bacterial community composition, we identified operational taxonomic units (OTUs), Families and Phyla with high heritability. The top associations (1-Mb windows) were located on 7 chromosomes. These regions were associated with the rumen microbiota in multiple ways; some (chromosome 19; position 3.0-4.0 Mb) are associated with closely related taxa (Prevotellaceae, Paraprevotellaceae, and RF16), some (chromosome 27; position 3.0-4.0 Mb) are associated with distantly related taxa (Prevotellaceae, Fibrobacteraceae, RF16, RFP12, S24-7, Lentisphaerae, and Tenericutes) and others (chromosome 23; position 0.0-1.0) associated with both related and unrelated taxa. The annotated genes associated with identified genomic regions suggest the associations observed are directed toward selective absorption of volatile fatty acids from the rumen to increase energy availability to the host. This study demonstrates that host genetics affects rumen bacterial community composition.