Letter: Modification of end-groups in DNA strand breaks of irradiated thymocytes during early repair.
Letter: Modification of end-groups in DNA strand breaks of irradiated thymocytes during early repair.
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信件：早期修复过程中受辐射胸腺细胞 DNA 链断裂端基的修饰。
DOI：
10.1080/09553007514550921
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
U. Hagen
中科院分区：
文献类型：
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作者：
M. Lennartz;T. Coquerelle;U. Hagen
2.材料和方法用10 MeV电子照射胸腺细胞，悬浮在Hank溶液中并用氧气或氮气鼓泡（参见Coquerelle，Bopp，Kessler和哈根1973）。为了在照射后孵育细胞，使用具有两个隔室的容器，通过水套保持在37 ℃。照射后，一个隔室（2ml）中的细胞在5秒内裂解，而另一个隔室（8ml）中的细胞保持在37 ℃并通入氧气2分钟以使修复反应能够进行（参见Modig，Edgren和Revesz 1974）。在照射后的不同时间，取1ml样品并裂解。从裂解的细胞中分离DNA，并通过Coquerelle等人所述的分析超离心法中的沉降测定双链断裂（dsb）、实际单链断裂（ssb）和碱诱导链断裂（alisb）的频率。（1973年）。在ssb和alisb的末端上，通过末端标记评估3'OH末端基团的频率。
2. Materials and methods Thymocytes were irradiated with 10 MeV electrons, suspended in Hank's solution and bubbled with oxygen or nitrogen (see Coquerelle, Bopp, Kessler and Hagen 1973). To incubate the cells after irradiation a vessel with two compartments was used kept at 37 C by a water-jacket. After irradiation, the cells in one compartment (2 ml) were lysed within 5 sec, while the cells in the other compartment (8 ml) remained at 37 C and were supplied with oxygen for 2 min to enable repair reaction (see Modig, Edgren and Revesz 1974). At various times after irradiation, 1 ml samples were taken and lysed. From the lysed cells DNA was isolated, and the frequency of double-strand breaks (dsb), of actual single-strand breaks (ssb) and alkali-induced strand breaks (alisb) was determined by means of sedimentation in the analytical ultracentrifuge as described by Coquerelle et al.(1973). On the terminals of the ssb and alisb, the frequency of 3'OH end-groups was evaluated by means of the terminal