Interaction of latex-insolubilized endotoxins with murine macrophages: phagocytic responses of endotoxin-responsive (C3HeB/FeJ) and -unresponsive (C3H/HeJ) macrophages in vitro.
Interaction of latex-insolubilized endotoxins with murine macrophages: phagocytic responses of endotoxin-responsive (C3HeB/FeJ) and -unresponsive (C3H/HeJ) macrophages in vitro.
复制标题
乳胶不溶性内毒素与小鼠巨噬细胞的相互作用：内毒素反应性 (C3HeB/FeJ) 和无反应性 (C3H/HeJ) 巨噬细胞的体外吞噬反应。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
D. Morrison
中科院分区：
文献类型：
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作者：
S. Lubinsky;P. Munkenbeck;D. Morrison
通过共价偶联多糖缺陷型S. miniR 595和富含多糖的E. coli 055：B5转化到羧化胶乳颗粒中。这些LPS-胶乳复合物的稳定性使用几种测定法测定，以检测在环境温度和升高的温度下孵育后的可溶性LPS。在体外培养不同时间后，检查了LPS应答C3 HeB/FeJ和LPS无应答C3 H/HeJ小鼠的驻留和巯基乙酸盐诱导的巨噬细胞吞噬LPS颗粒的能力。随着孵育时间的增加，巨噬细胞内的颗粒数量增加，证明了摄取。发现粗糙的多糖缺陷型LPS-乳胶颗粒比对照颗粒更容易被吞噬，而光滑的富含多糖的LPS颗粒比对照颗粒更不容易被吞噬。在本研究中使用的内毒素应答和无应答小鼠品系的巨噬细胞对颗粒的相对摄取速率中发现了定性相似的结果。
Insolubilized lipopolysaccharides (LPS) were prepared by covalently coupling LPS from polysaccharide-deficient S. minnesota R595 and polysaccharide-rich E. coli 055:B5 to carboxylated latex particles. The stability of these LPS-latex complexes was determined using several assays to detect soluble LPS following incubation at ambient and elevated temperatures. Resident and thioglycollate-elicited macrophages from both LPS-responder C3HeB/FeJ and LPS nonresponder C3H/HeJ mice were examined for their capacity to phagocytose the LPS particles following in vitro culture for various time periods. Uptake was demonstrated by an increase in the number of particles within the macrophages with increasing time of incubation. Rough polysaccharide-deficient LPS-latex particles were found to be more readily phagocytosed than control particles, whereas smooth polysaccharide-rich LPS particles were phagocytosed less readily than the controls. Qualitatively similar results were found in the relative rate of uptake of particles by the macrophages from the endotoxin-responsive and -unresponsive mouse strains used in this study.