Voltammetric biosensors for the determination of paracetamol at carbon nanotube modified pyrolytic graphite electrode
Voltammetric biosensors for the determination of paracetamol at carbon nanotube modified pyrolytic graphite electrode
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DOI：
10.1016/j.snb.2010.05.019
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发表时间：
2010-08-06
影响因子：
8.4
通讯作者：
Chatterjee, Sanghamitra
中科院分区：
文献类型：
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作者：
Goyal, Rajendra N.;Gupta, Vinod K.;Chatterjee, Sanghamitra
在磷酸盐缓冲溶液中，采用方波伏安法研究了扑热息痛在单壁碳纳米管（SWNT）修饰边缘平面热解石墨电极（EPPGE）上的伏安氧化行为。循环伏安法和方波伏安法研究表明，对乙酰氨基酚在电极表面的氧化通过两个电子的可逆步骤，并从根本上控制的吸附。除了半无限平面扩散外，还提出了纳米管修饰电极上薄层扩散的作用。单壁碳纳米管修饰的EPPGE的灵敏度比多壁碳纳米管修饰的EPPGE高2倍左右。对乙酰氨基酚在pH 7.2（mu = 0.5 M）下在类似于187 my处产生灵敏的氧化峰，用于在5-1000 nM范围内定量药物，在SWNT修饰的EPPGE上的检测限为2.9 x 10（-9）M。已报告生理学常见干扰物对扑热息痛电流响应的干扰作用。该方法已成功应用于药物制剂中对乙酰氨基酚的含量测定。所开发的方法，以确定对乙酰氨基酚给药后4小时获得的人尿样中的药物的适用性进行了说明。(C)2010 Elsevier B. V.保留所有权利。
The voltammetric oxidation of paracetamol on single-walled carbon nanotubes (SWNT) modified edge plane pyrolytic graphite electrode (EPPGE) was explored in phosphate buffer solution by using square wave voltammetry. Cyclic and square wave voltammetry studies indicated the oxidation of paracetamol at the electrode surface through a two-electron reversible step and fundamentally controlled by adsorption. Besides semi-infinite planar diffusion, the role of thin layer diffusion at nanotube modified electrodes is also suggested. The sensitivity at SWNT modified EPPGE is similar to 2 times more than that at MWNT modified EPPGE. Paracetamol gave a sensitive oxidation peak at similar to 187 my at pH 7.2 (mu = 0.5 M) which was used to quantitate the drug in the range of 5-1000 nM with a detection limit of 2.9 x 10(-9) M at SWNT modified EPPGE. The interfering effect of physiologically common interferents on the current response of paracetamol has been reported. The procedure was successfully applied for the assay of paracetamol in pharmaceutical formulations. The applicability of the developed method to determine the drug in human urine samples obtained after 4h of administration of paracetamol is illustrated. (C) 2010 Elsevier B.V. All rights reserved.