The in vitro antimicrobial activities of metabolites from lactobacillus strains on Candida species implicated in Candida vaginitis.
The in vitro antimicrobial activities of metabolites from lactobacillus strains on Candida species implicated in Candida vaginitis.
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发表时间：
2011-10
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通讯作者：
A. Ogunshe;M. A. Omotoso;Victoria B Bello
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作者：
A. Ogunshe;M. A. Omotoso;Victoria B Bello
背景：尼日利亚等发展中国家对女性泌尿生殖道乳酸菌种类及其作为阴道感染屏障的作用的研究有限。因此，本研究的目的是评估本地乳杆菌物种的临床生物治疗潜力。方法采用简单、易重现的定性和定量方法对抗菌代谢物的生产进行表征。采用改良琼脂斑点法和琼脂孔扩散法研究了抗菌乳杆菌对外阴阴道念珠菌相关念珠菌的体外抑制作用。结果20株阴道乳杆菌中乳酸、过氧化氢和双乙酰的最高含量分别为1.46 mg/L、1.36 mmol/L和1.72 mg/L。4名健康受试者乳酸最高水平为1.08 mg/L；过氧化氢，1.36 mmol/L；二乙酰，0.86 mg/L。这些物质的最大产量发生在72至120小时的孵育期间。患病受试者阴道中嗜酸乳杆菌、发酵乳杆菌、短乳杆菌、植物乳杆菌、干酪乳杆菌、德尔布鲁氏乳杆菌和简氏乳杆菌的体外抑菌活性最高为5.71%，而健康受试者阴道中嗜酸乳杆菌和植物乳杆菌的体外抑菌活性最高为57.1% ~ 68.6%。结论抗菌乳酸菌可作为外阴阴道念珠菌病的辅助生物治疗药物。
BACKGROUND Research from developing countries, such as Nigeria, on Lactobacillus species in the female urogenital tract and their role as a barrier to vaginal infection is limited. Therefore, the aim of this study was to assess the clinical biotherapeutic potential of indigenous Lactobacillus species. METHODS Antimicrobial metabolites production were characterised using simple and easily reproducible qualitative and quantitative methods. The in vitro inhibitory effect of Lactobacillus antimicrobials on vulvovaginal candidiasis-associated Candida species was investigated using modified agar spot and agar well-diffusion methods. RESULTS The maximum levels of lactic acid, hydrogen peroxide, and diacetyl from 20 vaginal Lactobacillus strains from diseased subjects were 1.46 mg/L, 1.36 mmol/L, and 1.72 mg/L respectively. From the 4 healthy subjects, the maximum level of lactic acid was 1.08 mg/L; hydrogen peroxide, 1.36 mmol/L; and diacetyl, 0.86 mg/L. The maximum productions of these substances occurred between 72 and 120 hours of incubation. The in vitro antagonistic activities of vaginal L. acidophilus, L. fermentum, L. brevis, L. plantarum, L. casei, L. delbrueckii, and L. jensenii from diseased subjects inhibited a maximum of 5.71% of the 35 Candida species tested, while vaginal L. acidophilus and L. plantarum from healthy subjects inhibited between 57.1% and 68.6% of Candida species in vitro. CONCLUSION Antimicrobial-producing lactobacilli can be considered as adjunct biotherapeutic candidates for the treatment of vulvovaginal candidiasis.