Efficacy of injectable praziquantel for elimination of trematode metacercariae in bluegills (Lepomis macrochirus) and quantification of parasite death by propidium iodide staining
Efficacy of injectable praziquantel for elimination of trematode metacercariae in bluegills (Lepomis macrochirus) and quantification of parasite death by propidium iodide staining
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DOI：
10.1007/s00436-017-5703-8
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发表时间：
2018-02-01
影响因子：
2
通讯作者：
Brewer, Matthew T.
中科院分区：
文献类型：
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作者：
Bader, Chris;Chelladurai, Jeba Jesudoss;Brewer, Matthew T.
地沟虫吸虫具有复杂的生命周期，控制这些扁虫可以通过消除中间宿主的未成熟寄生虫阶段来实现。在水产养殖系统中，吸虫囊蚴的存在会对鱼类健康产生负面影响，并导致经济损失。最小后蝶口虫是一种以蓝鳃太阳鱼（Lepomis macrochirus）为中间宿主的鸟类寄生虫，通常在用于娱乐目的的水道储存的鱼类中发现。在这项研究中，我们评估了注射吡喹酮对自然感染的蓝鳃鱼最小微囊蚴的杀伤作用。通过碘化丙啶染色和运动评估，我们发现5mg /kg肌肉注射对寄生虫有有效的杀灭作用。然而，直到治疗后第7天才出现明显的包囊死亡。实验结果表明，碘化丙啶染色法是检测处理过的鱼体内包虫病死亡的有效方法。该方法至少与客观运动评分一样敏感，并提供了寄生虫死亡的定量评估。未来涉及吡喹酮治疗鱼体内囊蚴病的研究可能需要在数周的时间内进行，以便准确评估寄生虫的杀灭效果，并将受益于使用碘化丙啶方法。
Digenean trematodes have complex life cycles and control of these flatworms can be accomplished by eliminating immature parasite stages from intermediate hosts. In aquaculture systems, presence of trematode metacercariae can negatively impact fish health and lead to economic losses. Posthodiplostomum minimum is a parasite of birds that uses bluegill sunfish (Lepomis macrochirus) as the intermediate host and is commonly found in fish used to stock waterways for recreational purposes. In this study, we evaluated killing of P. minimum metacercariae by injectable praziquantel in naturally infected bluegills. Using propidium iodide staining and motility assessment, we found that 5 mg/kg administered intramuscularly was effective for parasite killing. However, metacercarial death was not apparent until day 7 post-treatment. Our results demonstrated that propidium iodide staining is an effective method for detecting death in metacercariae recovered from treated fish. This method was at least as sensitive as objective motility scoring and provided quantitative assessment of parasite death. Future studies involving treatment of metacercariae in fish with praziquantel may need to be carried out over a period of weeks in order to accurately assess parasite killing and would benefit from using the propidium iodide method.