Immunoelectron microscopy shows a clustered distribution of NADPH oxidase components in the human neutrophil plasma membrane.
Immunoelectron microscopy shows a clustered distribution of NADPH oxidase components in the human neutrophil plasma membrane.
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免疫电镜显示人中性粒细胞质膜中 NADPH 氧化酶成分呈簇状分布。
DOI：
10.1002/jlb.61.3.303
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发表时间：
1997
影响因子：
5.5
通讯作者：
Hartwig,JH
中科院分区：
文献类型：
--
作者：
Wientjes,FB;Segal,AW;Hartwig,JH
The NADPH oxidase that produces superoxide in professional phagocytic cells is a flavocytochromebelectron transport chain in the membrane, a heterodimer of gp91phoxand p22phox, that is activated by a number of cytosolic proteins, including p47phoxp67phox, and the small GTP-binding protein p21rac, which translocate to the membrane and attach to the flavocytochrome on activation. The components of this oxidase were localized on the cytoplasmic surface of the plasma membrane of adherent unroofed neutrophils by immunolabeling. Components of the NADPH oxidase and p21racwere found together in punctate clusters occupying 0.03–0.1 μm2of the cytoplasmic surface of the plasma membrane where the density of labeling of the cytosolic components was increased after stimulation with phorbol myristate acetate.
