Functional expression of HGF and HGF receptor/c-met in adult human mesenchymal stem cells suggests a role in cell mobilization, tissue repair, and wound healing
Functional expression of HGF and HGF receptor/c-met in adult human mesenchymal stem cells suggests a role in cell mobilization, tissue repair, and wound healing
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DOI：
10.1634/stemcells.22-3-405
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发表时间：
2004-01-01
期刊：
影响因子：
5.2
通讯作者：
Jahnen-Dechent, W
中科院分区：
文献类型：
--
作者：
Neuss, S;Becher, E;Jahnen-Dechent, W
人间充质干细胞（hMSC）是具有多能性的成体干细胞。间充质干细胞分化为多种细胞类型的能力，以及它们高的体外扩增潜力，使这些细胞成为细胞移植和组织工程的有吸引力的治疗工具。hMSC被认为有助于组织再生，但控制其动员、渗透到血液和迁移到目标组织的信号在很大程度上是未知的。在这里，我们报道肝细胞生长因子（HGF）和同源受体HGFR/c-met在hMSC中表达，在RNA和蛋白质水平上。转化生长因子可下调HGF的表达。HGF刺激趋化迁移，但不刺激hMSC增殖。因此，HGF/c-met信号系统可能在组织再生的hMSC招募位点中起重要作用。HGF/c-met表达的调控在hMSC的组织工程和细胞治疗中可能是有益的。
Human mesenchymal stem cells (hMSC) are adult stem cells with multipotent capacities. The ability of mesenchymal stem cells to differentiate into many cell types, as well as their high ex vivo expansion potential, makes these cells an attractive therapeutic tool for cell transplantation and tissue engineering. hMSC are thought to contribute to tissue regeneration, but the signals governing their mobilization, diapedesis into the bloodstream, and migration into the target tissue are largely unknown. Here we report that hepatocyte growth factor (HGF) and the cognate receptor HGFR/c-met are expressed in hMSC, on both the RNA and the protein levels. The expression of HGF was downregulated by transforming growth factor beta. HGF stimulated chemotactic migration but not proliferation of hMSC. Therefore the HGF/c-met signaling system may have an important role in hMSC recruitment sites of tissue regeneration. The controlled regulation of HGF/c-met expression may be beneficial in tissue engineering and cell therapy employing hMSC.