Sequence‐based screening for self‐sufficient P450 monooxygenase from a metagenome library
Sequence‐based screening for self‐sufficient P450 monooxygenase from a metagenome library
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DOI：
10.1111/j.1365-2672.2006.03169.x
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发表时间：
2007-05
影响因子：
4
通讯作者：
B.S. Kim;S. Kim;J. Park;W. Park;K. Hwang;Y. Yoon;W.K. Oh;B.Y. Kim;J. Ahn
中科院分区：
文献类型：
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作者：
B.S. Kim;S. Kim;J. Park;W. Park;K. Hwang;Y. Yoon;W.K. Oh;B.Y. Kim;J. Ahn
目的：细胞色素 P450 单加氧酶 (CYP) 是氧化反应的有用催化剂。自给自足的 CYP 具有与 P450 结构域共价连接的还原结构域，以其强大的催化活性而闻名，具有作为生物催化剂的巨大潜力。为了扩大自给自足的 CYP 的遗传来源，我们设计了一种基于序列的筛选系统来在土壤宏基因组中识别它们。
Aims: Cytochrome P450 monooxygenases (CYPs) are useful catalysts for oxidation reactions. Self‐sufficient CYPs harbour a reductive domain covalently connected to a P450 domain and are known for their robust catalytic activity with great potential as biocatalysts. In an effort to expand genetic sources of self‐sufficient CYPs, we devised a sequence‐based screening system to identify them in a soil metagenome.