Core-shell SERS nanotags-based western blot.
Core-shell SERS nanotags-based western blot.
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DOI：
10.1016/j.talanta.2022.123888
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发表时间：
2022-09
期刊：
影响因子：
6.1
通讯作者：
Bing Liu;Hanyu Tang;Qian Liu;Wenwen Wang;Haitao Li;Shiya Zheng;Fei Sun;Xiangwei Zhao
中科院分区：
文献类型：
--
作者：
Bing Liu;Hanyu Tang;Qian Liu;Wenwen Wang;Haitao Li;Shiya Zheng;Fei Sun;Xiangwei Zhao
蛋白质印迹（WB）是生命科学中最常用的蛋白质鉴定方法，但其在低丰度蛋白质的精确定量检测方面仍面临巨大挑战。在这里，我们提出了一种新的表面增强拉曼散射为基础的蛋白质印迹（SERS-WB），以解决这一挑战。Sers纳米标签作为蛋白质的定量标记物，由金银核壳纳米粒子组成，尼罗蓝A（NBA）分子锚定在核壳界面。结果表明，SERS-WB对甘油醛-3-磷酸脱氢酶（GAPDH）蛋白的检测限为0.15 pg，线性动态范围为382 fg ~ 382 ng，具有良好的灵敏度。此外，由于纳米颗粒的聚集效应，硝酸纤维素（NC）膜上的目标蛋白可以直接通过比色信号进行识别，这大大简化了过程。这一战略的提出将为世行的技术创新带来新的思路。
Western blot (WB) is the most commonly used scheme for protein identification in life science, but it still faces great challenges in the accurate quantitative detection of low-abundance proteins. Here, we proposed a novel surface-enhanced Raman scattering-based Western blot (SERS-WB) to solve this challenge. SERS nanotags were used as quantitative labels of proteins, which were composed of gold-silver core-shell nanoparticles, and Nile blue A (NBA) molecules were anchored on the interface of the core and shell. The results show that the SERS-WB possessed excellent sensitivity with detection limit of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of 0.15 pg, as well as wide linear dynamic range (LDR) of 382 fg to 382 ng. In addition, the target protein on nitrocellulose (NC) membrane could be directly identified by colorimetric signal due to the aggregation effect of nanoparticles, which greatly simplifies the procedure. This as-proposed strategy will bring new thoughts to technological innovation of WB.