Nuclear access and action of notch in vivo
Nuclear access and action of notch in vivo
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DOI：
10.1016/s0092-8674(00)81193-9
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发表时间：
1998-05-15
期刊：
影响因子：
64.5
通讯作者：
Adachi, A
中科院分区：
文献类型：
--
作者：
Struhl, G;Adachi, A
果蝇Notch（N）基因编码一种保守的单程跨膜受体，其转导控制细胞命运的细胞外信号。在这里，我们提出的证据表明，Notch的细胞内结构域获得进入细胞核的配体响应，可能是通过一种机制，涉及蛋白水解裂解和释放的蛋白质的其余部分。此外，我们的研究结果表明，Notch的信号转导依赖于细胞内结构域的能力，特别是包含CDC10重复的部分，到达细胞核，并参与下游靶基因的转录激活。
The Drosophila Notch (N) gene encodes a conserved single-pass transmembrane receptor that transduces extracellular signals controlling cell fate. Here, we present evidence that the intracellular domain of Notch gains access to the nucleus in response to ligand, possibly through a mechanism involving proteolytic cleavage and release from the remainder of the protein. In addition, our results suggest that signal transduction by Notch depends on the ability of the intracellular domain, particularly the portion containing the CDC10 repeats, to reach the nucleus and to participate in the transcriptional activation of downstream target genes.