Reversible transdifferentiation of secretory epithelial cells into adipocytes in the mammary gland
Reversible transdifferentiation of secretory epithelial cells into adipocytes in the mammary gland
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DOI：
10.1073/pnas.0407647101
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发表时间：
2004-11-30
影响因子：
11.1
通讯作者：
Cinti, S
中科院分区：
文献类型：
--
作者：
Morroni, M;Giordano, A;Cinti, S
哺乳动物的乳房脂肪组织在怀孕期间被泌乳腺所取代；哺乳中断时会发生相反的过程。形态学和溴脱氧尿苷研究提供了间接证据，表明小鼠乳腺脂肪细胞在怀孕期间转化为分泌性上皮细胞，并在哺乳后恢复为脂肪细胞。通过使用 Cre-loxP 重组系统，我们发现乳清酸性蛋白 (WAP)-Cre/R26R 小鼠的乳腺（其中分泌性上皮细胞在怀孕期间表达 lacZ 基因）在复旧期间含有标记的脂肪细胞。相反，脂肪细胞P2-Cre/R26R小鼠在怀孕前对脂肪细胞进行标记，在怀孕期间含有标记的分泌性上皮细胞。我们得出的结论是，在怀孕和哺乳期成年小鼠的乳腺中发生可逆的脂肪细胞到上皮和上皮到脂肪细胞的转分化。
Mammalian breast adipose tissue is replaced by a milk-secreting gland during pregnancy; the reverse process takes place upon interruption of lactation. Morphological and bromodeoxyuridine studies provide indirect evidence that mouse mammary adipocytes transform into secretory epithelial cells during pregnancy and revert to adipocytes after lactation. By using the Cre-loxP recombination system we show that the mammary gland of whey acidic protein (WAP)-Cre/R26R mice, in which secretory epithelial cells express the lacZ gene during pregnancy, contains labeled adipocytes during involution. Conversely, adipocyte P2-Cre/R26R mice, in which adipocytes are labeled before pregnancy, contain labeled secretory epithelial cells during pregnancy. We conclude that reversible adipocyte-to-epithelium and epithelium-to-adipocyte transdifferentiation occurs in the mammary gland of adult mice during pregnancy and lactation.