Cytochalasin-B: effects on cytokinesis, glycogen and 3 H-D-gluconse incorporation.
Cytochalasin-B: effects on cytokinesis, glycogen and 3 H-D-gluconse incorporation.
复制标题
细胞松弛素-B：对胞质分裂、糖原和 3 H-D-葡萄糖掺入的影响。
作者：
J. W. Sanger;H. Holtzer
在低浓度细胞松弛素b培养基中生长的He La细胞的延时照片显示，在分裂过程中，沟沟正常进行，直到中体形成。直到那时，向心收缩的沟才被阻断。随着时间的推移，收缩的沟松弛，形成双核细胞。细胞松弛素b对细胞内糖原水平和3h - d -葡萄糖与糖原、糖蛋白和粘多糖的结合有明显的抑制作用。这些观察结果表明，对药物的敏感性可能更多地是由于干扰葡萄糖的摄取和利用，而不是由于细胞皮层中假定的可收缩微丝的破坏。
Time-lapse pictures of He La cells grown in a medium with low concentrations of Cytochalasin-B revealed that during division furrowing proceeds normally until the mid-body forms. Only then was the centripetal contraction of the furrow blocked. In time the contracted furrow relaxed, resulting in a binucleated cell. Cytochalasin-B exerted a profound depression on the level of glycogen in the cells and on the incorporation of 3H-D-glucose into glycogen, glycoprotein and mucopolysaccharides. These observations suggest that sensitivity to the drug may be due more to interference with glucose uptake and utilization than to disruption of putative contractile microfilaments in the cell cortex.