LAMINAR DISTRIBUTION OF CHOLINERGIC MARKERS IN NEOCORTEX - EFFECTS OF LESIONS
LAMINAR DISTRIBUTION OF CHOLINERGIC MARKERS IN NEOCORTEX - EFFECTS OF LESIONS
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DOI：
10.1002/jnr.490060506
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发表时间：
1981-01-01
影响因子：
4.2
通讯作者：
COYLE, JT
中科院分区：
文献类型：
--
作者：
JOHNSTON, MV;YOUNG, AC;COYLE, JT
突触前标记物的胆碱能和GABA能神经元在大鼠外侧新皮层的各层的分布确定与技术，其中部分增加的深度水平软膜表面上获得的冷冻阶段切片机和分析的标记物。胆碱乙酰转移酶和乙酰胆碱酯酶的具体活动有一个不均匀的分布在皮层与最低水平的活动与层II和III，而谷氨酸脱羧酶活性最高的层II，III和IV。胎儿引起的发育不全的皮质导致在胆碱乙酰转移酶和乙酰胆碱酯酶的活性在所有层中的谷氨酸脱羧酶的活性没有显着变化的富集。基底核-皮质胆碱能通路的损伤导致所有皮质层中乙酰胆碱酯酶和胆碱乙酰转移酶的活性显著降低，并消除了皮质内这些标记物的不均匀分布。乙酰胆碱酯酶活性显然与皮质胆碱能传入有关。支持皮质内胆碱能神经元的存在。
Distribution of presynaptic markers for cholinergic and GABAergic neurons in the various layers of the lateral neocortex of the rat was determined with a technique in which sections of increasing depth horizontal to the pial surface were obtained on a frozen-stage microtome and analyzed for the markers. Specific activities of choline acetyltransferase and acetylcholinesterase had an uneven distribution in the cortex with lowest levels of activity associated with lamina II and III, whereas glutamate decarboxylase activity was highest in lamina II, III and IV. Fetally-induced hypoplasia of the cortex resulted in an enrichment in both choline acetyltransferase and acetylcholinesterase activity in all layers with no significant change in the activity of glutamate decarboxylase. Lesion of the nucleus basalis-cortical cholinergic pathway resulted in marked reductions in the activities of acetylcholinesterase and choline acetyltransferase in all cortical layers and an elimination of the uneven distribution of these markers within the cortex. Acetylcholinesterase activity is apparently specifically associated with cortical cholinergic afferents. The existence of cortical intrinsic cholinergic neurons is supported.