Measurement of cytokines in sweat patches and plasma in healthy women: Validation in a controlled study
Measurement of cytokines in sweat patches and plasma in healthy women: Validation in a controlled study
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DOI：
10.1016/j.jim.2006.07.011
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发表时间：
2006-08-31
影响因子：
2.2
通讯作者：
Phillips, Terry M.
中科院分区：
文献类型：
--
作者：
Marques-Deak, Andrea;Cizza, Giovanni;Phillips, Terry M.
已通过ELISA在多种体液中检测到细胞因子。循环免疫亲和色谱（RIC）结合激光诱导荧光检测是一种高灵敏度和特异性的方法，可同时测定少量生物体液中的多种分析物。该方法已应用于血浆、宫颈分泌物和其他体液，但以前从未应用于汗液。本研究的目的是验证汗液中IL-1 α、IL-1 β、IL-6、TNF-α、IL-8和TGF-β测量的RIC方法。在9名健康女性的躯干上贴上两个汗贴，持续24小时，并在此期间的一个时间点收集血液。在血浆和汗液的配对样品中测量细胞因子。在汗液中检测到与血浆浓度相似的细胞因子。线性回归分析证实这些细胞因子的汗液水平占血浆水平变异的很大百分比：IL-1 alpha（R-2 = 0.70，p = 0.005），IL-1 β（R-2 = 0.79，p = 0.003）、IL-6（R-2 = 0.52，p = 0.03）、TNF-α（R-2 = 0.95，p < 0.0001）、IL-8（R-2 = 0.81，p = 0.001）和TGF-β（R-2 = 0.94，p = 0.0003）。这些发现表明，汗液中测量的细胞因子水平是循环水平的信息，并且汗液贴片与RIC结合代表了一种可行的非侵入性方法，用于随时间推移测量流动环境中的细胞因子。这种方法是不引人注目的，并要求最低限度的积极遵守的一部分，正在研究的主题，没有痛苦或压力。这种方法可以开启新一代的研究，以解决环境因素对各种不同环境中免疫反应的影响。由爱思唯尔公司出版
Cytokines have been detected by ELISA in a variety of body fluids. Recycling immunoaffinity chromatography (RIC) coupled with laser-induced fluorescence detection is a highly sensitive and specific method, which allows simultaneous measurements of many analytes in small volumes of biological fluids. This method has been applied to plasma, cervical secretions and other body fluids, but has not previously been applied to sweat. The aim of this study was to validate the RIC methodology in sweat for measurements of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IL-8 and TGF-beta. Two sweat patches were applied for 24 h on the torso, and blood was collected atone time point during this period in nine healthy women. Cytokines were measured in paired samples of plasma and sweat. Cytokines were detected in sweat in similar concentrations to plasma. Linear regression analysis confirmed that sweat levels of these cytokines accounted fora large percentages of variance in plasma levels: IL-1 alpha (R-2 = 0.70, p = 0.005), IL-1 beta (R-2 = 0.79, p = 0.003), IL-6 (R-2 = 0.52, p = 0.03), TNF-alpha (R-2 = 0.95, p < 0.0001), IL-8 (R-2 = 0.81, p = 0.001) and TGF-beta (R-2 = 0.94, p = 0.0003). These findings indicate that cytokine levels measured in sweat are informative of circulating levels and that sweat patches combined with RIC represents a viable non-invasive method to measure cytokines in ambulatory settings over time. This method is unobtrusive and requires minimal active compliance on the part of the subjects being studied, without pain or stress. This approach can open a new generation of studies to address the effects of environmental factors on immune responses in a wide range of different settings. Published by Elsevier B.V.