fMRI response to blue light delivery in the naive brain: Implications for combined optogenetic fMRI studies
fMRI response to blue light delivery in the naive brain: Implications for combined optogenetic fMRI studies
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DOI：
10.1016/j.neuroimage.2012.10.074
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发表时间：
2013-02-01
期刊：
影响因子：
5.7
通讯作者：
Lythgoe, Mark F.
中科院分区：
文献类型：
--
作者：
Christie, Isabel N.;Wells, Jack A.;Lythgoe, Mark F.
光遗传学和功能性磁共振成像（fMRI）的结合被称为光-fMRI。光遗传学利用基因工程将光敏致动蛋白引入细胞。功能性磁共振成像（fMRI）是一种专门的磁共振成像形式，涉及脑中血流和氧合的成像变化，与代谢活动的区域变化有关。本研究描述了一种方法学上的关注光传递到大脑的目的opto-fMRI的影响。我们发现，尽管没有光遗传学激活，但蓝光传递到幼稚大鼠大脑会引起深刻的fMRI反应。我们证明，这些功能磁共振成像的反应是依赖于激光功率，并表明激光引起显着的加热。我们确定了加热如何影响MR信号，导致NMR频移、以及T1和T2* 变化。这项研究引起了人们的注意，一个可能的混杂因素，必须考虑到当opto-fMRI实验的设计。(C)2012 Elsevier Inc. All rights reserved.
The combination of optogenetics and functional magnetic resonance imaging (fMRI) is referred to as opto-fMRI. Optogenetics utilises genetic engineering to introduce light sensitive actuator proteins into cells. Functional MRI (fMRI) is a specialist form of magnetic resonance imaging concerned with imaging changes in blood flow and oxygenation, linked to regional variation in metabolic activity, in the brain. This study describes a methodological concern regarding the effects of light delivery into the brain for the purposes of opto-fMRI. We show that blue light delivery to the naive rat brain causes profound fMRI responses, despite the absence of optogenetic activation. We demonstrate that these fMRI responses are dependent upon laser power and show that the laser causes significant heating. We identify how heating impacts upon the MR signal causing NMR frequency shifts,, and T1 and T2* changes. This study brings attention to a possible confounder which must be taken into account when opto-fMRI experiments are designed. (C) 2012 Elsevier Inc. All rights reserved.