Excitatory Cerebellar Nucleocortical Circuit Provides Internal Amplification during Associative Conditioning.
Excitatory Cerebellar Nucleocortical Circuit Provides Internal Amplification during Associative Conditioning.
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DOI：
10.1016/j.neuron.2016.01.008
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发表时间：
2016-02-03
期刊：
影响因子：
16.2
通讯作者：
De Zeeuw CI
中科院分区：
文献类型：
--
作者：
Gao Z;Proietti-Onori M;Lin Z;Ten Brinke MM;Boele HJ;Potters JW;Ruigrok TJ;Hoebeek FE;De Zeeuw CI
皮质和皮质下区域之间的闭环电路可以促进输出模式的精确性，但此类网络在小脑中的作用仍有待阐明。在这里，我们描述了在经典眨眼条件反射中从小脑核到小脑皮层的内部反馈的作用。我们发现插入核中的兴奋性输出神经元通过苔藓纤维向属于同一模块的小脑皮质区提供传出复制信号，触发颗粒细胞和高尔基体细胞的单突触反应并间接抑制浦肯野细胞。经过调节后，核皮质苔藓纤维末端的局部密度显着增加。条件动物中核皮质纤维的光遗传学激活和抑制分别增加和减少习得的眨眼反应的幅度。我们的数据表明，小脑的兴奋性核皮质闭环电路传递运动前信号的必然放电，并表明该电路在控制联想运动学习中具有放大作用。小脑核向小脑皮质提供模块化推论放电 核皮质传入神经具有独特的分子和超微结构特征 眨眼调节诱导核皮质苔藓纤维的结构可塑性 核皮质传入神经放大条件性眨眼反应的幅度 小脑核和皮质之间的闭环电路的作用尚不清楚。高等人。表明核皮质输入向颗粒层提供伴随放电，在眨眼条件作用下具有可塑性，并放大条件反应的幅度。
Closed-loop circuitries between cortical and subcortical regions can facilitate precision of output patterns, but the role of such networks in the cerebellum remains to be elucidated. Here, we characterize the role of internal feedback from the cerebellar nuclei to the cerebellar cortex in classical eyeblink conditioning. We find that excitatory output neurons in the interposed nucleus provide efference-copy signals via mossy fibers to the cerebellar cortical zones that belong to the same module, triggering monosynaptic responses in granule and Golgi cells and indirectly inhibiting Purkinje cells. Upon conditioning, the local density of nucleocortical mossy fiber terminals significantly increases. Optogenetic activation and inhibition of nucleocortical fibers in conditioned animals increases and decreases the amplitude of learned eyeblink responses, respectively. Our data show that the excitatory nucleocortical closed-loop circuitry of the cerebellum relays a corollary discharge of premotor signals and suggests an amplifying role of this circuitry in controlling associative motor learning. Cerebellar nuclei provide modular corollary discharge to the cerebellar cortex Nucleocortical afferents have unique molecular and ultrastructural features Eyeblink conditioning induces structural plasticity of nucleocortical mossy fibers Nucleocortical afferents amplify the amplitude of conditioned eyeblink responses The role of the closed-loop circuitry between cerebellar nuclei and cortex is unknown. Gao et al. show that nucleocortical inputs provide corollary discharges to the granular layer, are plastic upon eyeblink conditioning, and amplify the amplitude of conditioned responses.