FRIGIDA-related genes are required for the winter-annual habit in Arabidopsis
FRIGIDA-related genes are required for the winter-annual habit in Arabidopsis
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DOI：
10.1073/pnas.0306778101
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发表时间：
2004-03-02
影响因子：
11.1
通讯作者：
Amasino, RM
中科院分区：
文献类型：
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作者：
Michaels, SD;Bezerra, IC;Amasino, RM
在温带气候中，冬季漫长的寒冷温度是冬季一年生和两年生植物的季节性标志。在这些植物中，冬季前开花受阻。在拟南芥中，FRIGIDA(FRI)基因的自然变异是决定快速循环开花习性与冬季开花习性的主要因素。在拟南芥的冬季一年生材料中，FRi活性通过花抑制基因C(FLC)的上调而阻止开花。相反，大多数快速开花材料含有FRi的零等位基因。通过对冬季一年生品系进行突变筛选，我们已经确定了一个FRIGIDA Like 1(FRL1)基因座，它是FRI上调FLC所特需的。克隆FRL1发现了一个基因，其预测的蛋白质序列与Fri的同源性为23%。尽管序列相似，但FRI和FRL1不具有冗余功能。FRi和FRL1属于拟南芥中的一个七人基因家族，FRi、FRL1和至少一个额外的家族成员FRIGIDA like 2(FRL2)属于这个家族的一个分支，这是拟南芥越冬习性所必需的。
In temperate climates, the prolonged cold temperature of winter serves as a seasonal landmark for winter-annual and biennial plants. In these plants, flowering is blocked before winter. In Arabidopsis thaliana, natural variation in the FRIGIDA (FRI) gene is a major determinate of the rapid-cycling vs. winter-annual flowering habits. In winter-annual accessions of Arabidoosis, FRI activity blocks flowering through the up-regulation of the floral inhibitor FLOWERING LOCUS C(FLC). Most rapid-flowering accessions, in contrast, contain null alleles of FRI. By performing a mutant screen in a winter-annual strain, we have identified a locus, FRIGIDA LIKE 1 (FRL1), that is specifically required for the up-regulation of FLC by FRI. Cloning of FRL1 revealed a gene with a predicted protein sequence that is 23% identical to FRI. Despite sequence similarity, FRI and FRL1 do not have redundant functions. FRI and FRL1 belong to a seven-member gene family in Arabidopsis, and FRI, FRL1, and at least one additional family member, FRIGIDA LIKE 2 (FRL2), are in a clade of this family that is required for the winter-annual habit in Arabidopsis.